foodborne pathogen

食源性病原体
  • 文章类型: Journal Article
    确保食品安全,特别是对于弱势群体,像婴儿和幼儿,需要识别和优先考虑食物链中的潜在危害。我们之前开发了一个基于网络的决策支持系统(DSS),通过结构化的五步过程来识别婴幼儿食品中的特定微生物危害(MHs)。本研究通过引入系统风险排名(RR)步骤,以七个标准对MH风险进行排名来进一步框架:过程生存,再污染,增长机会,膳食准备,危害食品协会证据,欧盟婴幼儿的食物消费习惯,和MH严重性。每个标准都给出了半定量或定量的评分或风险值,通过三种汇总方法为最终的MH风险计算做出贡献:半定量风险评分,半定量风险值,超越多准则决策分析(MCDA)。为了验证标准和排名方法,我们进行了一项案例研究,对婴儿配方奶粉中的MH风险进行排名,比较了三种风险排序方法的结果,并根据专家意见对排名结果进行了额外评估,以确保其准确性。结果表明,三种方法具有很强的一致性,持续排名非伤寒沙门氏菌和克罗恩杆菌属。和产志贺毒素的大肠杆菌是婴儿配方食品中最大的MH风险,微小的偏差。当MHs在初始危险识别步骤之后进行排序时,这三种方法都产生了几乎相同的MH排名,加强排名步骤和选定标准的可靠性。值得注意的是,与风险评分方法相比,风险值和MCDA方法提供了更多信息的MH排名.将风险值和风险评分方法实施到在线工具中,称为微生物危害风险调查决策支持系统(Mira-DSS),可在https://foodmicrologywur上获得。shinyapps.io/微生物_危害_RAnking/。总之,我们的框架可以对MH风险进行排名,促进干预比较和资源分配,以减轻婴儿食品中的MH风险,具有对更广泛食品类别的潜在适用性。
    Ensuring food safety, particularly for vulnerable groups, like infants and young children, requires identifying and prioritizing potential hazards in food chains. We previously developed a web-based decision support system (DSS) to identify specific microbiological hazards (MHs) in infant and toddler foods through a structured five-step process. This study takes the framework further by introducing systematic risk ranking (RR) steps to rank MH risks with seven criteria: process survival, recontamination, growth opportunity, meal preparation, hazard-food association evidence, food consumption habits of infants and toddlers in the EU, and MH severity. Each criterion is given a semi-quantitative or quantitative score or risk value, contributing to the final MH risk calculation via three aggregation methods: semi-quantitative risk scoring, semi-quantitative risk value, and outranking multi-criteria decision analysis (MCDA). To validate the criteria and ranking approaches, we conducted a case study to rank MH risks in infant formula, compared the results of the three risk ranking methods, and additionally evaluated the ranking results against expert opinions to ensure their accuracy. The results showed strong agreement among the three methods, consistently ranking Salmonella non-Typhi and Cronobacter spp. and Shiga-toxin-producing Escherichia coli as the top MH risks in infant formulae, with minor deviations. When MHs were ranked after an initial hazard identification step, all three methods produced nearly identical MH rankings, reinforcing the reliability of the ranking steps and the selected criteria. Notably, the risk value and MCDA methods provided more informative MH rankings compared to the risk scoring method. The risk value and risk scoring methods were implemented into an online tool, called the MIcrobiological hazards risk RAnking decision support system (Mira-DSS), available at https://foodmicrobiologywur.shinyapps.io/MIcrobial_hazards_RAnking/. In conclusion, our framework enables the ranking of MH risks, facilitating intervention comparisons and resource allocations to mitigate MH risks in infant foods, with potential applicability to broader food categories.
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  • 文章类型: Journal Article
    已发现肠出血性大肠杆菌O157:H7(EHECO157:H7)和产肠毒素大肠杆菌(ETEC)容易在黄瓜(CucumissativusL.)上形成生物膜,对即食蔬菜的安全构成重大风险。本研究旨在评估裂解噬菌体vB_EcoM_SQ17(SQ17)对黄瓜上EHECO157:H7和ETEC生物膜的有效性。这里,我们评估了噬菌体SQ17对EHECO157:H7和ETEC菌株在各种表面上形成和减少生物膜的功效,包括聚苯乙烯,聚-D-赖氨酸预涂层薄膜,还有鲜切的黄瓜,在不同的温度。噬菌体SQ17显著抑制ETEC生物膜的形成,在37°C时将粘附细胞的数量减少0.15logCFU/mL。通过SEM观察,用噬菌体SQ17处理也显着减少了建立的生物膜中粘附细胞的数量。此外,噬菌体SQ17在孵育24小时后,在37°C下有效地将EHECO157:H7和ETEC生物膜的生物量降低了54.8%以上。噬菌体处理后,在4°C和25°C下,在黄瓜上的生物膜中,粘附的EHECO157:H7细胞的活力降低了1.37logCFU/片和0.46logCFU/片,分别。同样,在4°C和25°C下,黄瓜上的生物膜中ETEC细胞的活力降低了1.07logCFU/片和0.61logCFU/片,分别。这些发现表明,噬菌体SQ17有望作为根除黄瓜上致病性大肠杆菌生物膜的潜在策略。
    Enterohemorrhagic Escherichia coli O157:H7 (EHEC O157:H7) and Enterotoxigenic E. coli (ETEC) have been found to readily develop biofilms on cucumber (Cucumis sativus L.), presenting a significant risk to the safety of ready-to-eat vegetables. This study aimed to assess the effectiveness of the lytic bacteriophage vB_EcoM_SQ17 (SQ17) against EHEC O157:H7 and ETEC biofilms on cucumber. Here, we evaluated the efficacy of phage SQ17 on the formation and reduction of biofilms formed by EHEC O157:H7 and ETEC strains on various surfaces, including polystyrene, poly-d-lysine precoated films, and fresh-cut cucumber, at different temperatures. Phage SQ17 significantly inhibited ETEC biofilm formation, reducing the number of adhered cells by 0.15 log CFU/mL at 37 °C. Treatment with phage SQ17 also significantly decreased the number of adhered cells in established biofilms via SEM observation. Moreover, phage SQ17 effectively reduced the biomass of EHEC O157:H7 and ETEC biofilms by over 54.8 % at 37 °C after 24 h of incubation. Following phage treatment, the viability of adhered EHEC O157:H7 cells decreased by 1.37 log CFU/piece and 0.46 log CFU/piece in biofilms on cucumber at 4 °C and 25 °C, respectively. Similarly, the viability of ETEC cells decreased by 1.07 log CFU/piece and 0.61 log CFU/piece in biofilms on cucumber at 4 °C and 25 °C, respectively. These findings suggest that phage SQ17 shows promise as a potential strategy for eradicating pathogenic E. coli biofilms on cucumber.
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  • 文章类型: Journal Article
    单核细胞增生李斯特菌(L.单核细胞增多症)是一种食源性病原体,可引起人类和其他动物的李斯特菌病。具有LPXTG基序的表面蛋白在单核细胞增生李斯特菌的毒力中具有重要作用。Lmo0159是一种这样的蛋白质,但对它在单核细胞增生李斯特菌毒力中的作用知之甚少,运动性,和生物膜的形成。这里,我们构建并表征了lmo0159的缺失突变体(Δlmo0159)。我们不仅分析了生物膜形成的能力,运动性,附件,和不同细胞类型的细胞内生长,还有LD50;小鼠肝脏中的细菌负荷,脾,脾和大脑;毒力基因的表达;以及攻击后小鼠的存活时间。结果表明,通过显微镜检查,Δlmo0159菌株的生物膜交联密度低于WT。生物膜形成和毒力基因的表达在生物膜状态下也降低。随后,Δlmo0159在培养基中的生长和运动增强。相反,单核细胞增生李斯特菌的生长和运动性在细胞和小鼠水平上都被Δlmo0159减弱。在细胞层面,Δlmo0159减少斑块大小;加速划痕愈合;并减弱粘连的效率,入侵,和猪肠道上皮细胞(SIEC)的细胞内增殖,RAW264.7,小鼠脑微血管内皮细胞(mBMEC),和人脑微血管内皮细胞(hCMEC/D3)。毒力基因的表达也受到抑制。在鼠标级别,Δlmo0159菌株的LD50是WT菌株的100.97倍。Δlmo0159菌株在肝脏和脾脏中的细菌负荷低于WT菌株。在腹腔感染的小鼠模型中,lmo0159基因的缺失显著延长了小鼠的存活时间,表明lmo0159缺失突变体也表现出降低的毒力。因此,我们的研究确定lmo0159是单核细胞增生李斯特菌LPXTG蛋白中的一种新型毒力因子。
    Listeria monocytogenes (L. monocytogenes) is a foodborne pathogen that causes listeriosis in humans and other animals. Surface proteins with the LPXTG motif have important roles in the virulence of L. monocytogenes. Lmo0159 is one such protein, but little is known about its role in L. monocytogenes virulence, motility, and biofilm formation. Here, we constructed and characterized a deletion mutant of lmo0159 (∆lmo0159). We analyzed not only the capacity of biofilm formation, motility, attachment, and intracellular growth in different cell types but also LD50; bacterial load in mice\'s liver, spleen, and brain; expression of virulence genes; and survival time of mice after challenge. The results showed that the cross-linking density of the biofilm of ∆lmo0159 strain was lower than that of WT by microscopic examination. The expression of biofilm-formation and virulence genes also decreased in the biofilm state. Subsequently, the growth and motility of ∆lmo0159 in the culture medium were enhanced. Conversely, the growth and motility of L. monocytogenes were attenuated by ∆lmo0159 at both the cellular and mouse levels. At the cellular level, ∆lmo0159 reduced plaque size; accelerated scratch healing; and attenuated the efficiency of adhesion, invasion, and intracellular proliferation in swine intestinal epithelial cells (SIEC), RAW264.7, mouse-brain microvascular endothelial cells (mBMEC), and human-brain microvascular endothelial cells (hCMEC/D3). The expression of virulence genes was also inhibited. At the mouse level, the LD50 of the ∆lmo0159 strain was 100.97 times higher than that of the WT strain. The bacterial load of the ∆lmo0159 strain in the liver and spleen was lower than that of the WT strain. In a mouse model of intraperitoneal infection, the deletion of the lmo0159 gene significantly prolonged the survival time of the mice, suggesting that the lmo0159 deletion mutant also exhibited reduced virulence. Thus, our study identified lmo0159 as a novel virulence factor among L. monocytogenes LPXTG proteins.
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  • 文章类型: Journal Article
    细菌科细菌家族包括从动物和相关食品中分离的物种。此外,这些物种已经在其他生态位被发现,包括水。一些物种,特别是Butzleri杆菌和低温嗜氧杆菌,已从人类临床病例中分离出来,并与胃肠道症状有关。抗生素耐药菌株的存在是公众健康的问题,考虑到可能的人畜共患病和食源性感染是由含有对抗生素治疗有抗性的细菌的污染食物引起的。这篇综述旨在强调糖杆菌属中抗生素耐药性的重要性。从几个来源分离,包括有关该细菌已显示出抗药性的抗生素类别的信息。弓形虫。表现出广泛的抗生素耐药性,包括几个抗生素抗性基因。抗生素抗性基因组性状包括抗生素靶蛋白中的外排泵和突变。文献显示高比例的糖杆菌属。具有多重耐药性。然而,文献中的研究主要集中在对Butzleri和A.cryaerophilus的抗生素耐药性的评估上,因为这些物种经常从各种来源中分离出来。这些方面强调了将重点放在可能从几种来源分离的几种拟杆菌属物种上的研究的必要性。
    The Arcobacteraceae bacterial family includes species isolated from animals and related food products. Moreover, these species have been found in other ecological niches, including water. Some species, particularly Arcobacter butzleri and Arcobacter cryaerophilus, have been isolated from human clinical cases and linked to gastrointestinal symptoms. The presence of antibiotic-resistant strains is a concern for public health, considering the possible zoonoses and foodborne infections caused by contaminated food containing bacteria resistant to antibiotic treatments. This review aims to highlight the importance of antibiotic resistance in Arcobacter spp. isolates from several sources, including information about antibiotic classes to which this bacterium has shown resistance. Arcobacter spp. demonstrated a wide spectrum of antibiotic resistance, including several antibiotic resistance genes. Antibiotic resistance genomic traits include efflux pumps and mutations in antibiotic target proteins. The literature shows a high proportion of Arcobacter spp. that are multidrug-resistant. However, studies in the literature have primarily focused on the evaluation of antibiotic resistance in A. butzleri and A. cryaerophilus, as these species are frequently isolated from various sources. These aspects underline the necessity of studies focused on several Arcobacter species that could potentially be isolated from several sources.
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  • 文章类型: Journal Article
    空肠弯曲杆菌是全球食源性疾病的主要原因。开发针对空肠弯曲杆菌的替代抗菌策略,这项研究设计并优化了具有药用真菌无柄灵芝细胞内成分的金属纳米颗粒(NPs)的绿色合成,以提供所需的还原剂和稳定剂。通过透射电子显微镜和动态光散射对NP进行表征,准球形NP的氧化铜NP的尺寸为2.9±0.9nm,银NP的尺寸为14.7±0.6nm。表面电荷评估显示,氧化铜和银NP的zeta电位为-21.0±6.5mV和-24.4±7.9mV,分别。NPs对空肠弯曲杆菌的生长抑制通过附着到外部细胞膜和随后的细胞内内化而发生,并导致6μg/mL的银NP和10μg/mL的氧化铜NP的最小抑制浓度。另一方面,观察到由银和铜NP引起的不同的ROS产生。总之,这项研究首次证明了使用绿色合成与药用真菌G.无柄产生有效抑制空肠弯曲菌生长的金属NPs,为传统的抗菌药物使用提供可持续和有效的方法。
    Campylobacter jejuni is a major cause of global foodborne illnesses. To develop alternative antimicrobial strategies against C. jejuni, this study designed and optimized the green synthesis of metallic nanoparticles (NPs) with intracellular components of the medicinal fungus Ganoderma sessile to provide the needed reducing and stabilizing agents. NPs were characterized by transmission electron microscopy and dynamic light scattering, and the quasi-spherical NPs had sizes of 2.9 ± 0.9 nm for the copper oxide NPs and 14.7 ± 0.6 nm for the silver NPs. Surface charge assessment revealed zeta potentials of -21.0 ± 6.5 mV and -24.4 ± 7.9 mV for the copper oxide and silver NPs, respectively. The growth inhibition of C. jejuni by the NPs occurred through attachment to the outer cell membrane and subsequent intracellular internalization and resulted in minimum inhibitory concentrations of the silver NPs at 6 µg/mL and copper oxide NPs at 10 µg/mL. On the other hand, a differential ROS production caused by silver and copper NPs was observed. In summary, this research presents the first demonstration of using green synthesis with the medicinal fungus G. sessile to produce metallic NPs that effectively inhibit C. jejuni growth, providing a sustainable and effective approach to the traditional use of antimicrobials.
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  • 文章类型: Journal Article
    这项研究的重点是噬菌体的分离和表征,该噬菌体具有针对蜡状芽孢杆菌的产毒素和多重耐药菌株的比活性(B.cereuss.s.)。利用双层琼脂技术,十个不同的样品产生了六个噬菌体。最有前途的噬菌体,vB_BceS-M2是基于其宽宿主范围和针对各种蜡状芽孢杆菌s.s.菌株的强裂解活性来选择的。噬菌体vB_BceS-M2具有56,482bp的环状双链DNA基因组。这种噬菌体在很宽的温度和pH值范围内表现出稳定性,这对其在食品基质中的潜在应用至关重要。噬菌体vB_BceS-M2和乳酸链球菌素的联合作用,一种广泛使用的抗菌肽,研究了增强食品中对蜡状芽孢杆菌的抗微生物功效。结果表明,乳酸链球菌素与噬菌体具有协同作用和联合作用,有可能克服肉汤中噬菌体抗性细菌的生长。此外,在各种液体和固体食品基质中进行了实际应用,包括全脂和脱脂牛奶,煮米饭,奶酪,还有冷冻肉丸,在4和25°C噬菌体vB_BceS-M2,单独或与乳酸链球菌素组合,降低了全脂牛奶以外的食物中蜡样芽孢杆菌的生长速度。噬菌体和乳酸链球菌素的组合显示出有望开发有效的抗微生物干预措施,以抵抗食品中的产毒和抗生素抗性蜡状芽孢杆菌。
    This study focused on the isolation and characterization of bacteriophages with specific activity against toxin-producing and multidrug-resistant strains of Bacillus cereus sensu stricto (B. cereus s. s.). Ten different samples yielded six bacteriophages by utilizing the double-layer agar technique. The most promising phage, vB_BceS-M2, was selected based on its broad host range and robust lytic activity against various B. cereus s. s. strains. The phage vB_BceS-M2 had a circular double-stranded DNA genome of 56,482 bp. This phage exhibited stability over a wide range of temperatures and pH values, which is crucial for its potential application in food matrices. The combined effect of phage vB_BceS-M2 and nisin, a widely used antimicrobial peptide, was investigated to enhance antimicrobial efficacy against B. cereus in food. The results suggested that nisin showed synergy and combined effect with the phage, potentially overcoming the growth of phage-resistant bacteria in the broth. Furthermore, practical applications were conducted in various liquid and solid food matrices, including whole and skimmed milk, boiled rice, cheese, and frozen meatballs, both at 4 and 25 °C. Phage vB_BceS-M2, either alone or in combination with nisin, reduced the growth rate of B. cereus in foods other than whole milk. The combination of bacteriophage and nisin showed promise for the development of effective antimicrobial interventions to counteract toxigenic and antibiotic-resistant B. cereus in food.
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  • 文章类型: Journal Article
    由于食品安全对人类健康和整体福祉的直接影响,食品安全是全球关注的焦点。在食品加工环境中,食源性病原体的生物膜形成带来了一个重大问题,因为它导致持续和高水平的食品污染,从而损害食品的质量和安全性。因此,有效去除食品加工环境中的生物被膜,确保食品安全势在必行。不幸的是,传统的清洁方法不足以充分去除生物膜,它们甚至可能进一步污染设备和食物。有必要开发替代方法来应对食品工业中的这一挑战。解决生物膜相关问题的一个有前途的策略是生物膜分散,这代表了生物膜开发的最后一步。这里,我们讨论了食源性病原体的生物膜分散机制,并阐明了生物膜分散如何用于控制和减轻生物膜相关问题。通过揭示这些方面,我们的目标是提供有价值的见解和解决方案,以有效解决食品工业中的生物膜污染问题,从而提高食品安全和确保消费者的福祉。
    Food safety is a critical global concern due to its direct impact on human health and overall well-being. In the food processing environment, biofilm formation by foodborne pathogens poses a significant problem as it leads to persistent and high levels of food contamination, thereby compromising the quality and safety of food. Therefore, it is imperative to effectively remove biofilms from the food processing environment to ensure food safety. Unfortunately, conventional cleaning methods fall short of adequately removing biofilms, and they may even contribute to further contamination of both equipment and food. It is necessary to develop alternative approaches that can address this challenge in food industry. One promising strategy in tackling biofilm-related issues is biofilm dispersion, which represents the final step in biofilm development. Here, we discuss the biofilm dispersion mechanism of foodborne pathogens and elucidate how biofilm dispersion can be employed to control and mitigate biofilm-related problems. By shedding light on these aspects, we aim to provide valuable insights and solutions for effectively addressing biofilm contamination issues in food industry, thus enhancing food safety and ensuring the well-being of consumers.
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  • 文章类型: Journal Article
    艰难梭菌及其内生孢子具有食源性病原体的特征,已在食物链的几个阶段被检测到。在宿主肠道生态失衡的情况下,艰难梭菌可增殖并引起肠道感染。多种食物来源因素可以显著改变宿主的肠道生态系统,包括白酒的消费。然而,饮用白酒引起的肠道生态变化是否会增加艰难梭菌入侵和感染的风险,还有待了解。在这项研究中,将艰难梭菌细胞暴露于两种市售的白酒以评价白酒对艰难梭菌细胞的作用并通过小鼠模型进行验证。结果表明,白酒能有效抑制艰难梭菌的生长和生物膜的产生,下调tcdA和tcdB毒力基因的表达水平,但上调产孢子基因Spo0A的表达水平,提高了孢子的产量,以及增加艰难梭菌细胞对Caco-2细胞的粘附。小鼠模型表明,摄入白酒促进艰难梭菌孢子的侵袭和感染,对盲肠组织造成损害,伴随着肠道脂质载体蛋白2(Lcn-2)和TcdA毒素蛋白水平的增加。同时,胆酸升高,而脱氧胆酸减少。这项研究首次发现了白酒摄入量与艰难梭菌孢子入侵和感染之间的可能联系。
    Clostridioides difficile and its endospores possess the characteristics of a foodborne pathogen and have been detected at several stages in the food chain. In the presence of an imbalance in host intestinal ecology, C. difficile can proliferate and cause intestinal infections. Multiple food source factors can substantially alter the host\'s gut ecosystem, including the consumption of baijiu. However, it remains to be known whether the gut ecological changes induced by the consumption of baijiu increase the risk of C. difficile invasion and infection. In this study, C. difficile cells were exposed to two commercially available baijiu to evaluate the effect of baijiu on C. difficile cells and to verify through a mouse model. The results showed that baijiu effectively inhibited the growth and biofilm production of C. difficile, downregulated the expression levels of tcdA and tcdB virulence genes but upregulated the expression level of spore-producing genes Spo0A, enhanced the spore production, as well as increased C. difficile cell adhesion to Caco-2 cells. The mouse model showed that the intake of baijiu promoted the invasion and infection of C. difficile spores, causing damage to the cecum tissue, accompanied by an increase in the gut lipid carrier protein-2 (Lcn-2) and TcdA toxin protein levels. Simultaneously, cholic acid was elevated, whereas deoxycholic acid was decreased. This study is the first to find a possible link between baijiu intake and C. difficile spore invasion and infection.
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  • 文章类型: Journal Article
    产气荚膜梭菌是引起食物中毒和动物肠炎的主要厌氧病原体之一。随着抗生素耐药性的上升和农业中使用抗生素生长促进剂(AGP)的限制,梭菌肠炎和食物沾染已变得更加普遍。用标准培养方法确认检测费时费力,有必要开发现场快速检测工具。在这项研究中,重组酶聚合酶扩增(RPA)和侧流生物传感器(LFB)的组合用于目视检测鸡肉和牛奶中的产气荚膜梭菌。
    针对产气荚膜梭菌的plc基因设计了两组引物,以及引物的扩增效率和特异性。引物的选择产生在其上设计探针的扩增片段。将探针与侧流生物传感器(LFB)组合。优化了RPA-LFB法的反应时间和温度,并对测定的灵敏度进行了评估。选择了几种常见的食源性致病菌,对所建立的方法进行特异性测试。用不同浓度(1×102CFU/mL至1×106CFU/mL)的产气荚膜梭菌人工接种鸡肉和牛奶样品,比较了RPA-LFB法和PCR法的检测效率。RPA-LFB可以在20分钟内完成,并且可以通过LFB测试条直观地读取结果。RPA-LFB具有可接受的特异性和100pg的最低检测限。/μL的核酸样品。能够在1×104CFU/mL和1×103CFU/mL的最低浓度下稳定检测鸡肉和牛奶中的产气荚膜梭菌污染,分别。
    总而言之,RPA-LFB具有特异性和敏感性。这是一个快速的,食品中产气荚膜梭菌的检测方法简单易行,适用于现场检测工作。
    UNASSIGNED: Clostridium perfringens is one of the major anaerobic pathogen causing food poisoning and animal enteritis. With the rise of antibiotic resistance and the restrictions of the use of antibiotic growth promoting agents (AGPs) in farming, Clostridium enteritis and food contamination have become more common. It is time-consuming and labor-intensive to confirm the detection by standard culture methods, and it is necessary to develop on-site rapid detection tools. In this study, a combination of recombinase polymerase amplification (RPA) and lateral flow biosensor (LFB) was used to visually detect C. perfringens in chicken meat and milk.
    UNASSIGNED: Two sets of primers were designed for the plc gene of C. perfringens, and the amplification efficiency and specificity of the primers. Selection of primers produces an amplified fragment on which the probe is designed. The probe was combined with the lateral flow biosensor (LFB). The reaction time and temperature of RPA-LFB assay were optimized, and the sensitivity of the assay was assessed. Several common foodborne pathogens were selected to test the specificity of the established method. Chicken and milk samples were artificially inoculated with different concentrations (1 × 102 CFU/mL to 1 × 106 CFU/mL) of C. perfringens, and the detection efficiency of RPA-LFB method and PCR method was compared. RPA-LFB can be completed in 20 min and the results can be read visually by the LFB test strips. The RPA-LFB has acceptable specificity and the lowest detection limit of 100 pg./μL for nucleic acid samples. It was able to stably detect C. perfringens contamination in chicken and milk at the lowest concentration of 1 × 104 CFU/mL and 1 × 103 CFU/mL, respectively.
    UNASSIGNED: In conclusion, RPA-LFB is specific and sensitive. It is a rapid, simple and easy-to-visualize method for the detection of C. perfringens in food and is suitable for use in field testing work.
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  • 文章类型: Journal Article
    目的:关于基因组数据库在公共卫生中的应用已经写了很多。在食品安全中,这些数据库包含来自两种类型的分离株的数据-来自患者的数据(即,临床)和非临床来源(例如,食品制造环境)。来自这些来源的分离株之间的遗传匹配代表了感兴趣的信号。我们调查了三个大型基因组数据库(单核细胞增生李斯特菌,大肠杆菌,和沙门氏菌)和较小的Cronobacter数据库;这些数据库是NCBI(国家生物技术信息中心)病原体检测项目的一部分。
    结果:目前,单核细胞增生李斯特菌的临床分离株与非临床分离株的匹配率为33%,46%为沙门氏菌,和7%的大肠杆菌。这些匹配率与几个数据库特征相关,包括生物体的多样性,数据库大小,和非临床生物样品的比例。通过逻辑回归建模匹配率显示出相对较好的性能。我们的预测模型说明了用非临床分离株填充数据库以更好地识别临床样品的匹配的重要性。此类信息应有助于公共卫生官员优先考虑监测策略,并显示填充新兴数据库的关键需求(例如,SakazakiiCronobacter).
    OBJECTIVE: Much has been written about the utility of genomic databases to public health. Within food safety these databases contain data from two types of isolates-those from patients (i.e., clinical) and those from non-clinical sources (e.g., a food manufacturing environment). A genetic match between isolates from these sources represents a signal of interest. We investigate the match rate within three large genomic databases (Listeria monocytogenes, Escherichia coli, and Salmonella) and the smaller Cronobacter database; the databases are part of the Pathogen Detection project at NCBI (National Center for Biotechnology Information).
    RESULTS: Currently, the match rate of clinical isolates to non-clinical isolates is 33% for L. monocytogenes, 46% for Salmonella, and 7% for E. coli. These match rates are associated with several database features including the diversity of the organism, the database size, and the proportion of non-clinical BioSamples. Modeling match rate via logistic regression showed relatively good performance. Our prediction model illustrates the importance of populating databases with non-clinical isolates to better identify a match for clinical samples. Such information should help public health officials prioritize surveillance strategies and show the critical need to populate fledgling databases (e.g., Cronobacter sakazakii).
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