背景:蜱传疾病在全球范围内对动物生产造成重大的经济损失,无形体病和Theileriosis与最大的损失有关。然而,有关病原体在埃及南部家养动物群中的传播鲜为人知。因此,在这项研究中,我们的目的是确定无性体的患病率,TheileriaOvis,和埃及南部绵羊和山羊的Theilerialestoquardi通过血液测试,并对绵羊中检测到的针对特定基因的A.Ovis进行分子表征。
结果:我们收集了埃及南部卢克索省300只绵羊和山羊(n=150/种)的血液样本,并分析了它们是否存在A.Ovis,通过常规和巢式PCR筛选靶向msp4和msp5,18SrRNA,和裂殖子表面蛋白基因。对于A.Ovis140/300样品(46.66%)总体呈阳性,绵羊和山羊的阳性样本为90/150(60%)和50/150(33.33%),分别。A.Ovis的两个主要表面蛋白基因,使用从绵羊和山羊血液样品中提取的DNA对msp4和msp5进行测序,用于系统发育分析和基因分型。msp4基因序列显示没有显著的遗传多样性,与其他国家的A.Ovis菌株的数据形成对比。对于T.Lestoquardi,8/150(5.33%)绵羊样本呈阳性,但山羊样本无阳性(0%)。对于T.Ovis,32/150(21.33%)样本在绵羊中呈阳性,但山羊样本无阳性(0%)。针对T.lestoquardi的裂殖子表面蛋白基因和T.ovis的小亚基核糖体RNA基因的测序显示,在研究中没有显着的遗传多样性,另一个对比来自其他国家的A.Ovis菌株的数据。
结论:这项研究提供了有关A.ovis的系统发育和分子分类的有价值的数据,在埃及南部的绵羊和山羊中发现的T.Ovis和T.lestoquardi。它也代表了基于特定裂殖子表面蛋白基因在埃及南部绵羊中检测和分子表征T.lestoquardi的第一份报告,从而为埃及南部这种病原体的分子鉴定提供了有价值的数据。
BACKGROUND: Tick-borne diseases cause economically significant losses to animal production globally, and anaplasmosis and theileriosis are associated with the greatest losses. However, the spread of the relevant pathogens in flocks of domesticated animals in southern Egypt is little understood. Accordingly, in this study, we aimed to determine the prevalences of Anaplasma ovis, Theileria ovis, and Theileria lestoquardi in southern Egyptian sheep and goats through blood tests, and to make a molecular characterization of the A. ovis detected in sheep targeting a specific gene.
RESULTS: We collected blood samples collected from 300 sheep and goats (n=150 /species) in Luxor Province in southern Egypt, and analyzed them for the presence of A. ovis, T. ovis and T. lestoquardi with screening by conventional and nested PCR targeting the msp4 and msp5, 18S rRNA, and merozoite surface protein genes. For A. ovis 140/300 samples (46.66%) were positive overall, with 90/150 (60%) and 50/150 (33.33%) positive samples in sheep and goats, respectively. Two major surface protein genes of A. ovis, msp4 and msp5, were sequenced using DNA extracted from sheep and goat blood samples, for phylogenetic analysis and genotyping. The msp4 gene sequence revealed no significant genetic diversity, to contrast to data on A. ovis strains from other countries. For T. lestoquardi, 8/150 (5.33%) samples were positive in sheep, but no samples were positive in goats (0%). For T. ovis, 32/150 (21.33%) samples were positive in sheep, but no samples were positive in goats (0%). Sequencing targeting the merozoite surface protein gene for T. lestoquardi and the small subunit ribosomal RNA gene for T. ovis revealed no significant genetic diversity in the study, another contrast to data on A. ovis strains from other countries.
CONCLUSIONS: This study provides valuable data on phylogenetic and molecular classifications of A. ovis, T. ovis and T. lestoquardi found in southern Egyptian sheep and goats. It also represents the first report on detection and molecular characterization of T. lestoquardi in southern Egyptian sheep based on the specific merozoite surface protein gene, thus providing valuable data for molecular characterization of this pathogen in southern Egypt.