PDF(Pubmed)
Abstract：
Raising small ruminants is the main source of income for farmers in Pakistan especially in rural areas of Dera Ghazi Khan in Punjab. Despite having large sheep population, the prevalence of intra-erythrocytic protozoa, Theileria (T.) lestoquardi, has never been reported from this area. This study was conducted to fill this knowledge gap and 333 blood samples of apparently healthy small ruminants (168 sheep and 165 goats) along with their epidemiological data were collected from Dera Ghazi Khan district during August till November 2022. The polymerase chain reaction (PCR) analysis amplified a 785 base pair amplicon specific for the Merozoite surface antigen (ms 1-2) gene of T. lestoquardi in 2 out of the 168 (3.3%) sheep blood samples, while no goat blood sample out of 165 was found to be infected with T. lestoquardi. DNA sequencing confirmed the presence of Theileria lestoquardi in both samples and phylogenetic analysis revealed that these amplicon resembled the partial ms 1-2 gene sequences detected in small ruminants from Pakistan, India Iran and Egypt. All the studied epidemiological factors (age, sex, breed, size of herd, dogs with herd, composition of herd, size of herd and Tick burden on sheep) were not found associated with the prevalence of T. lestoquardi. In conclusion, this study reports a low prevalence of T. lestoquardi infection in the Dera Ghazi Khan District of Punjab, Pakistan. The data generated from this work will help pave the way for the prophylactic detection and control of ovine and caprine theileriosis in the region.
摘要：
饲养小反刍动物是巴基斯坦农民的主要收入来源，尤其是旁遮普省德拉加齐汗的农村地区。尽管有大量的绵羊，红细胞内原生动物的患病率，Theileria(T.)lestoquardi，这个地区从来没有报道过。这项研究旨在填补这一知识空白，并于2022年8月至11月从DeraGhaziKhan地区收集了333份看起来健康的小反刍动物（168只绵羊和165只山羊）的血液样本及其流行病学数据。聚合酶链反应（PCR）分析在168个（3.3％）绵羊血液样品中的2个中扩增了对M.lestoquardi的Merozoite表面抗原（ms1-2）基因特异的785个碱基对扩增子，而在165份山羊血液样本中没有发现感染了利斯托夸迪。DNA测序证实了两个样本中都存在西里氏菌，系统发育分析表明，这些扩增子类似于巴基斯坦小反刍动物中检测到的部分ms1-2基因序列，印度伊朗和埃及。所有研究的流行病学因素(年龄，性别,品种,牛群的大小，有牛群的狗,群的组成，未发现牛群的大小和绵羊的Tick负担）与沙口孢菌的患病率相关。总之,这项研究报告在旁遮普省的德拉加齐汗地区，巴基斯坦。这项工作产生的数据将有助于为该地区绵羊和山羊的预防性检测和控制铺平道路。
