背景:DEAD-box蛋白(DDX)是DDXRNA解旋酶家族的成员,在RNA代谢中发挥多种功能,细胞周期,肿瘤发生,信号通路,和生育能力,特别是在哺乳动物中。然而,近年来DDXs在昆虫中的生物学功能尚未完全解决,引起了越来越多的关注。Laodelphoxstriatellus(半翅目)是一种臭名昭著的水稻害虫,以水稻汁液为食并传播植物病毒。在这项研究中,我们的目的是阐明DDXs在纹状体乳杆菌中的功能特征,并利用潜在的目标基因开发害虫控制策略。
结果:在这项研究中,我们表征了LsDDX6,LsDDX47和LsDDX51在飞虱中的表达模式,并分析了它们的保守基序。发现这些基因在检查的所有组织和发育阶段都有表达,在卵巢中观察到明显更高的转录水平。敲除LsDDX6,LsDDX47和LsDDX51导致若虫明显的致死表型和成人卵巢发育异常。此外,在纹状体乳杆菌中总共鉴定出27个DDX,大多数DDX在卵巢中高表达,结构分析结果表明,所有DDX都具有DDX家族特有的9个基序。
结论:三种DDXRNA解旋酶(LsDDX6、LsDDX47和LsDDX51)对于纹状体乳杆菌的存活和繁殖都是必需的。考虑到在纹样乳杆菌中鉴定出的27个DDX的总数,它们可能是应用于这种破坏性害虫的基于RNAi的控制的有希望的候选者。©2024化学工业学会。
BACKGROUND: DEAD-box protein (DDX) is a member of the DDX RNA helicase family that exerts multiple functions in RNA metabolism, cell cycle, tumorigenesis, signal pathway, and fertility, particularly in mammals. Nevertheless, the biological functions of DDXs in insects have not been fully resolved and attracted increasing attention these years. Laodelphax striatellus (Hemiptera) is a notorious rice pest through feeding on rice sap and transmitting plant viruses. In this study, we aim to elucidate the functional characterization of DDXs in L. striatellus, and to exploit potential target genes for the development of pest control strategies.
RESULTS: In this study, we characterized the expression patterns of LsDDX6, LsDDX47, and LsDDX51 in planthoppers and analyzed their conserved motifs. These genes were found to be expressed in all tissues and developmental stages examined, with significantly higher transcript levels observed in the ovary. Knockdown of LsDDX6, LsDDX47, and LsDDX51 resulted in an obvious lethal phenotype in nymphs and abnormal ovarian development in adults. Furthermore, a total of 27 DDXs were identified in L. striatellus, and most DDXs were highly expressed in ovary and structure analysis result revealed that all of the DDXs possessed nine motifs that were unique to the DDX family.
CONCLUSIONS: The three DDX RNA helicases (LsDDX6, LsDDX47, and LsDDX51) are essential for both survivorship and reproduction in L. striatellus. Considering a total number of 27 DDXs identified in L. striatellus, they might serve as promising candidates for application in RNAi-based control of this destructive pest. © 2024 Society of Chemical Industry.