双酚S(BPS)广泛用于制造产品,增加心血管疾病的风险。肥胖和BPS之间的关联对心脏预后的影响仍然未知。将雄性C57BL/6小鼠分为标准饮食(SC;15kJ/g),标准饮食+BPS(SCB),高脂饮食(HF;21kJ/g),和高脂饮食+BPS(HFB)。超过12周,两组通过饮用水(剂量:25μg/Kg/天)和/或HF饮食暴露于BPS。我们评估:体重(BM),总胆固醇,收缩压(SBP),左心室(LV)质量,和心脏重塑。在SCB组中,BM,总胆固醇,与SC组相关,SBP增加。在HF和HFB组中,这些参数高于SC和SCB组.左心室质量和壁厚增加证明了心肌肥厚。与SC组相比,所有组的ANP蛋白表达。只有HFB组比SCB和HF组有更厚的LV壁,与SC和SCB组相比,心肌细胞面积增加。关于心脏纤维化,SCB,HF,和HFB组呈现较高的间质胶原面积,TGFβ,而α-SMA蛋白表达高于SC组。仅HF和HFB组的血管周围胶原面积比SC组增加。较高的IL-6,TNFα,和CD11c卵白表达在一切组均比SC组证明的炎症。与SC组相比,所有组的CD36和PPARα蛋白表达均升高,但只有HF和HFB组比SC组促进心脏脂肪变性,perilipin5蛋白表达增加。单独BPS暴露促进病理性同心性肥大的心脏重塑,纤维化,和炎症。当与BPS相关时,饮食诱导的重塑会加剧,有明显的肥大,除了纤维化,炎症,和脂质积累。
Bisphenol S (BPS) is widely used in the manufacture products and increase the risk of cardiovascular diseases. The effect of the association between obesity and BPS on cardiac outcomes is still unknown. Male C57BL/6 mice were divided into standard chow diet (SC; 15 kJ/g), standard chow diet + BPS (SCB), high-fat diet (HF; 21 kJ/g), and high-fat diet + BPS (HFB). Over 12 weeks, the groups were exposed to BPS through drinking water (dose: 25 μg/Kg/day) and/or a HF diet. We evaluated: body mass (BM), total cholesterol, systolic blood pressure (SBP), left ventricle (LV) mass, and cardiac remodeling. In the SCB group, BM, total cholesterol, and SBP increase were augmented in relation to the SC group. In the HF and HFB groups, these parameters were higher than in the SC and SCB groups. Cardiac hypertrophy was evidenced by augmented LV mass and wall thickness, and ANP protein expression in all groups in comparison to the SC group. Only the HFB group had a thicker LV wall than SCB and HF groups, and increased cardiomyocyte area when compared with SC and SCB groups. Concerning cardiac fibrosis, SCB, HF, and HFB groups presented higher interstitial collagen area, TGFβ, and α-SMA protein expression than the SC group. Perivascular collagen area was increased only in the HF and HFB groups than SC group. Higher IL-6, TNFα, and CD11c protein expression in all groups than the SC group evidenced inflammation. All groups had elevated CD36 and PPARα protein expression in relation to the SC group, but only HF and HFB groups promoted cardiac steatosis with increased perilipin 5 protein expression than the SC group. BPS exposure alone promoted cardiac remodeling with pathological concentric hypertrophy, fibrosis, and inflammation. Diet-induced remodeling is aggravated when associated with BPS, with marked hypertrophy, alongside fibrosis, inflammation, and lipid accumulation.