背景:医疗保健相关的感染造成了巨大的公共卫生负担,导致发病率,死亡率,住院时间延长,以及巨大的社会和经济成本。免疫功能低下的患者医院感染的风险更高。
目的:这项在乌吉达的MohammedVI大学医院进行的前瞻性研究旨在评估免疫抑制病房与双人住院病房相比表面和空气的微生物生态。
方法:在微流Alpha的辅助下,采用沉降法和碰撞法进行了微生物空气纯度测试。沉降法使用穆勒·辛顿和5%的人血,促进受污染的尘埃颗粒的自由下落。所采用的收集程序设定为每Im310分钟。对于表面采样,拭子取自25cm2的表面。将拭子立即转交给微生物实验室。我们对菌落进行了宏观和微观鉴定,然后使用BDphoenixTM系统进行明确的生化鉴定。通过在MullerHinton培养基上的琼脂扩散以及最小抑制浓度的测定来评估抗生素敏感性。
结果:结果显示,保护隔离室内的细菌数量减少,与标准的医院房间相反。我们注意到凝固酶阴性葡萄球菌和芽孢杆菌属的优势。金黄色葡萄球菌和曲霉属,医疗保健相关感染中的常见病原体,在保护性隔离室中明显缺席。这些发现强调了医院环境在医疗保健相关感染传播中的关键作用。
结论:保护性隔离室对微生物污染进行了有效控制,抗性细菌越来越少。该研究强调了空气处理系统在防止机会性感染传播方面的重要性。我们的研究强调了微生物清洁度在预防医院感染中的关键作用。
BACKGROUND: Healthcare-associated infections pose a significant public health burden, leading to morbidity, mortality, prolonged hospital stays, and substantial social and economic costs. Immunocompromised patients are at a heightened risk of nosocomial infections.
OBJECTIVE: This prospective study conducted at Mohammed VI University Hospital of Oujda aimed to assess the microbial ecology of surfaces and air in an immunosuppressed patient room compared to a double hospitalization room.
METHODS: Microbiological air purity tests were conducted employing both the sedimentation method and the collision method with the assistance of Microflow Alpha. The sedimentation method used Mueller Hinton with 5% human blood, facilitating the free fall of contaminated dust particles. The collection program employed was set for 10 minutes per 1 m3. For surface sampling, swabs were taken from a 25 cm2 surface. The swabs were immediately forwarded to the Microbiology Laboratory. We carried out both macroscopic and microscopic identification of colonies, followed by definitive biochemical identification using the BD phoenixTM system. Antibiotic susceptibility was assessed through agar diffusion on Muller Hinton medium coupled with the determination of the minimum inhibitory concentration.
RESULTS: The results revealed a decreased bacterial count within the protective isolation room, in contrast to the standard hospital room. We noted the predominance of coagulase-negative Staphylococcus spp and Bacillus spp. Staphylococcus aureus and Aspergillus spp, common pathogens in healthcare-associated infections, were notably absent in the protective isolation room. The findings underline the pivotal role of hospital environments in the transmission of healthcare-associated infections.
CONCLUSIONS: The protective isolation room demonstrated effective control of microbial contamination, with fewer and less resistant germs. The study highlighted the significance of air treatment systems in preventing the spread of opportunistic infections. Our study underscored the critical role of microbiological cleanliness in preventing nosocomial infections.