背景:胎盘血管生成不良与几种妊娠并发症有关,包括胎儿生长受限(FGR),这导致低出生体重(LBW)婴儿在成年期具有生长障碍和代谢障碍的高风险。最近使用合胞苷敲除小鼠的研究显示胎盘血管形成的生长明显中断。由ERVW-1基因编码的Syncytin-1,被认为在胎盘血管生成中起作用,但其与LBW婴儿胎盘中血管内皮生长因子(VEGF)等其他促血管生成因子的关系尚未确定。通过了解FGR的机制,未来可以采取更积极的预防和治疗措施。本研究旨在检测ERVW-1、促血管生成基因VEGF及其受体(FLT-1)的表达,和缺氧诱导因子-1(HIF-1)在LBW胎盘,并研究这些基因在LBW婴儿胎盘中表达的关系。
方法:从胎盘组织中提取总RNA。总RNA用作cDNA合成模板,然后是qRT-PCR。ERVW-1、VEGF、用线性回归分析FLT-1和HIF-1基因的表达。
结果:具有LBW和正常出生体重(NBW)婴儿的母亲的年龄和体重指数没有显着差异。ERVW-1在LBW胎盘中的表达低于NBW胎盘,但是VEGF,FLT-1和HIF-1表达较高。ERVW-1与HIF-1、VEGF呈负相关。
结论:LBW婴儿胎盘中ERVW-1的低表达可能导致胎盘血管生成受损,并可能导致缺氧。
Poor placental angiogenesis is associated with several pregnancy complications including fetal growth restriction (FGR), which causes low birth weight (LBW) babies to have a high risk of growth disorders and metabolic disorders in adulthood. Recent research using syncytin knock-out mice showed significant disruption in the growth of placental vascularization. Syncytin-1 which encoded by ERVW-1 gene, is proposed to have a role in placental angiogenesis, but its relationship with other proangiogenic factors such as vascular endothelial growth factor (VEGF) in the placenta of LBW babies has not yet been determined. By knowing the mechanisms of FGR, more proactive preventive and therapeutic measures can be taken in the future. This study aimed to determine the expression of ERVW-1, proangiogenic gene VEGF and its receptor (FLT-1), and hypoxia inducible factor-1 (HIF-1) in LBW placentas, and investigate the relationship between these genes\' expression in the placenta of LBW babies.
Total RNA was extracted from placental tissue. Total RNA is used as a cDNA synthesis template, followed by qRT-PCR. Correlations of ERVW-1, VEGF, FLT-1 and HIF-1 genes\' expression were analyzed by linear regression.
The age and body mass index of mothers with LBW and normal birth weight (NBW) babies were not significantly different. ERVW-1 expression in LBW placentas was lower than in NBW placentas, but VEGF, FLT-1 and HIF-1 expressions were higher. ERVW-1 was negatively correlated with HIF-1 and VEGF.
Low expression of ERVW-1 in the placenta of LBW babies may result in impaired placental angiogenesis and possibly lead to hypoxia.