■糖尿病心脏并发症的全球患病率一直在增加,目前用于治疗糖尿病(DM)的一些药物无法缓解这种并发症。
■这项研究确定了巴西坚果(Bertholletiaexcelsa)和二甲双胍对果糖/链脲佐菌素(STZ)诱导的2型糖尿病大鼠糖尿病心肌病(DCM)的影响,并使用气相色谱-质谱法和傅立叶变换红外表征了巴西坚果50%乙醇提取物中的生物活性化合物。
■诱发2型DM后,将30只雄性白化病Wistar大鼠分为五组,每组六只大鼠,并按以下方式处理:第1组(对照)和第2组(糖尿病对照)大鼠接受大鼠颗粒和蒸馏水;第3组(糖尿病巴西坚果)接受大鼠颗粒和巴西坚果提取物(100mg/kg,口服)溶解在蒸馏水中,第4组(糖尿病+二甲双胍)接受二甲双胍(100mg/kg,口服)溶解在蒸馏水中,而第5组(糖尿病+巴西坚果+二甲双胍)口服巴西坚果(100mg/kg)和溶解在蒸馏水中的二甲双胍(100mg/kg)。这项研究持续了6周。使用的巴西坚果的剂量是从我们对不同浓度的巴西坚果提取物的最小治疗剂量的初步研究中选择的。
■STZ给药诱导胰岛素抵抗,高血糖症,体重减轻,血脂异常,氧化应激,炎症,凋亡,哺乳动物雷帕霉素靶的改变,丝裂原活化蛋白激酶,心功能标志物(肌酸激酶MB,乳酸脱氢酶,和天冬氨酸氨基转氨酶),和糖尿病控制的心脏组织学,用巴西坚果和二甲双胍治疗后得到改善,但是他们的联合治疗比单一治疗更好。
■这项研究表明,巴西坚果含有几种生物活性化合物,支持其生物学特性以及作为二甲双胍补充疗法的候选药物,可以减轻大鼠DM引起的心脏并发症。
UNASSIGNED: The global prevalence of diabetic heart complication has been on the increase, and some of the drugs that are currently used to treat diabetes mellitus (DM) have not been able to mitigate this complication.
UNASSIGNED: This study determines the effect of Brazil nut (Bertholletia excelsa) and metformin on diabetic cardiomyopathy (DCM) in fructose/streptozotocin (STZ)-induced type 2 diabetic rats and also characterizes using Gas Chromatography Mass Spectrophotometry and Fourier Transform Infrared the bioactive compounds in 50% aqueous ethanol extract of Brazil nut.
UNASSIGNED: After inducing type 2 DM, 30 male albino Wistar rats were separated into five groups that comprised of six rats per group, and they were treated as follows: groups 1 (Control) and 2 (Diabetic control) rats received rat pellets and distilled water; group 3 (Diabetic + Brazil nut) received rat pellets and Brazil nut extract (100 mg/kg, orally) dissolved in distilled water, group 4 (Diabetic + metformin) received metformin (100 mg/kg, orally) dissolved in distilled water, while group 5 (Diabetic + Brazil nut + metformin) received oral administrations of Brazil nut (100 mg/kg) and metformin (100 mg/kg) dissolved in distilled water. This study lasted for 6 weeks. The dose of Brazil nut used was selected from our pilot study on the minimum therapeutic dose of different concentrations of Brazil nut extract.
UNASSIGNED: STZ administration induced insulin resistance, hyperglycemia, loss of weight, dyslipidemia, oxidative stress, inflammation, apoptosis, alteration of mammalian target of rapamycin, mitogen-activated protein kinase, heart function markers (creatine kinase MB, lactate dehydrogenase, and aspartate amino transaminase), and heart histology of the diabetic control, which was ameliorated after treatment with Brazil nut and metformin, but their combined treatment was better than the single treatments.
UNASSIGNED: This study shows that Brazil nut contains several bioactive compounds that support its biological properties as well as its candidature as a complementary therapy to metformin in mitigating cardiac complications arising from DM in rats.