尽管文献中有关于不同颜色的发光二极管(LED)对不同生物的影响的研究,关于这些影响如何随温度升高而变化的信息有限。在这项研究中,蓝色的影响,绿色,红色和白色LED灯对斑马鱼早期发育过程的影响(Daniorerio(Hamilton,1822))进行了全面调查。此外,模拟全球变暖,研究了温度升高一度如何影响这一过程。为此,斑马鱼胚胎,将其放置在4hpf(受精后小时)的孵化器中,其内部分为四个区域,在三个不同的温度(28、29和30°C)下保持120h。选择保持在黑暗环境中的组作为对照。对照组的温度也以与其他组相同的速率升高。结果表明,在暴露期结束时,温度和浅色导致身体畸形增加。幼虫脑中HSP70和8-OHdG生物标志物的组织病理学损伤和免疫阳性信号,增加氧自由基,凋亡细胞和脂质在整个身体的积累,运动活动的增加,心率和血流量下降,并检测到超过30种代谢物水平的显著变化。此外,已经确定许多代谢途径受到影响,尤其是谷胱甘肽,维生素B6和嘧啶代谢。此外,据观察,温度升高一度会使这种负面影响恶化。结论是,蓝光是最接近对照组的光,并且比其他颜色的光危害小。研究表明,蓝光产生的结果与对照组中的结果最相似。
Although there are studies in the literature on the effects of different coloured light-emitting diodes (LEDs) on different organisms, there is limited information on how these effects change with temperature increase. In this study, the effects of blue, green, red and white LED lights on the early development process of zebrafish (Danio rerio (Hamilton, 1822)) were comprehensively investigated. In addition, to simulate global warming, it was examined how a one-degree temperature increase affects this process. For this purpose, zebrafish embryos, which were placed at 4 hpf (hours post fertilization) in an incubator whose interior was divided into four areas, were kept at three different temperatures (28, 29 and 30 °C) for 120 h. The group kept in a dark environment was chosen as the control. The temperature of the control group was also increased at the same rate as the other groups. The results showed that at the end of the exposure period, temperature and light colour caused an increase in body malformations. Histopathological damage and immunopositive signals of HSP 70 and 8-OHdG biomarkers in larval brains, increase in free oxygen radicals, apoptotic cells and lipid accumulation throughout the body, increase in locomotor activity, decrease in heart rate and blood flow, and significant changes in more than thirty metabolite levels were detected. In addition, it has been determined that many metabolic pathways are affected, especially glutathione, vitamin B6 and pyrimidine metabolism. Moreover, it has been observed that a one-degree temperature increase worsens this negative effect. It was concluded that blue light was the closest light to the control group and was less harmful than other light colours. The study revealed that blue light produced results that were most similar to those seen in the control group.