陈年大蒜,通过在高温和受控湿度下加热生大蒜(大蒜)一段时间获得,具有广泛的生物活性,但其在溃疡性结肠炎中的作用及其机制尚未完全阐明。我们研究了陈年大蒜(AG)中的生物活性成分,并探讨了口服AG对DSS诱导的小鼠结肠炎的影响。结果表明,衰老过程中抗氧化功能上调,抗炎和抗微生物化合物,如二氢咖啡酸,5-乙酰水杨酸,Verticine,S-烯丙基-L-半胱氨酸和D-岩藻糖。口服AG明显缓解结肠炎,减少结肠损伤,增强抗氧化和抗炎作用。大肠杆菌和马链球菌在与帕金森病密切相关的结肠炎小鼠的结肠中显著富集,细菌入侵上皮细胞,氧气素生物合成,和血红素生物合成,但是由于Akkermansia粘液的丰度增加,结肠中的AG介导的明显改变,乳酸杆菌。L-YJ,短双歧杆菌,Blautiawexlerae,脱硫微生物。P100A,并观察到了丘陵乳杆菌。接下来,我们证明口服AG重建结肠微生物组显着增加短链脂肪酸如乙酸的产生,丙酸,异丁酸,和异戊酸。这项研究提供了第一个数据,表明口服AG以肠道微生物群依赖的方式改善结肠炎症。我们的发现为AG介导的结肠炎缓解和AG作为调节肠道微生物群预防和治疗结肠炎的功能性食物提供了新的见解。
Aged
garlic, obtained by heating raw
garlic (Allium sativum) under high temperature and controlled humidity for a period, possesses a wide range of bioactivities, but its role in ulcerative colitis and its mechanism are not fully elucidated. We investigated the bioactive constituents in aged
garlic (AG) and explored the effect of oral AG delivery on DSS-induced murine colitis. The results revealed that the aging process up-regulated anti-oxidative, anti-inflammatory and anti-microbial compounds such as dihydrocaffeic acid, 5-acetylsalicylic acid, verticine, S-allyl-L-cysteine and D-fucose. Oral AG obviously alleviated colitis, reducing colon damage and enhancing anti-oxidative and anti-inflammatory effects. Escherichia coli and Streptococcus equinus dramatically were enriched in the colon of mice with colitis that were strongly associated with Parkinson\'s disease, bacterial invasion of epithelial cells, aerobactin biosynthesis, and heme biosynthesis, but a distinct AG-mediated alteration in the colon due to increasing abundance of Akkermansia muciniphila, Lactobacillus sp. L-YJ, Bifidobacterium breve, Blautia wexlerae, Desulfomicrobium sp. P100A, and Lentilactobacillus hilgardii was observed. Next, we demonstrated that colonic microbiome reconstruction by oral AG significantly increased the production of short-chain fatty acids such as acetic acid, propionic acid, isobutyric acid, and isovaleric acid. This study provides the first data indicating that oral AG ameliorates colonic inflammation in a gut microbiota-dependent manner. Our findings provide novel insights into the AG-mediated remission of colitis and AG as a functional food for modulating the gut microbiota to prevent and treat colitis.