PDF(Pubmed)
Abstract：
UNASSIGNED: The bZIP genes (bZIPs) are essential in numerous biological processes, including development and stress responses. Despite extensive research on bZIPs in many plants, a comprehensive genome-wide analysis of bZIPs in garlic has yet to be undertaken.
UNASSIGNED: In this study, we identified and classified 64 AsbZIP genes (AsbZIPs) into 10 subfamilies. A systematic analysis of the evolutionary characteristics of these AsbZIPs, including chromosome location, gene structure, conserved motifs, and gene duplication, was conducted. Furthermore, we also examined the nucleotide diversity, cis-acting elements, and expression profiles of AsbZIPs in various tissues and under different abiotic stresses and hormone treatments.
UNASSIGNED: Our findings revealed that gene replication plays a crucial role in the expansion of AsbZIPs, with a minor genetic bottleneck observed during domestication. Moreover, the identification of cis-acting elements suggested potential associations of AsbZIPs with garlic development, hormone, and stress responses. Several AsbZIPs exhibited tissue-preferential and stress/hormone-responsive expression patterns. Additionally, Asa7G01972 and Asa7G01379 were notably differentially expressed under various stresses and hormone treatments. Subsequent yeast two-hybridization and yeast induction experiments validated their interactions with Asa1G01577, a homologue of ABI5, reinforcing their importance in hormone and abiotic stress responses. This study unveiled the characteristics of the AsbZIP superfamily and lays a solid foundation for further functional analysis of AsbZIP in garlic.
UNASSIGNED: In this study, we identified and classified 64 AsbZIP genes (AsbZIPs) into 10 subfamilies. A systematic analysis of the evolutionary characteristics of these AsbZIPs, including chromosome location, gene structure, conserved motifs, and gene duplication, was conducted. Furthermore, we also examined the nucleotide diversity, cis-acting elements, and expression profiles of AsbZIPs in various tissues and under different abiotic stresses and hormone treatments.
UNASSIGNED: Our findings revealed that gene replication plays a crucial role in the expansion of AsbZIPs, with a minor genetic bottleneck observed during domestication. Moreover, the identification of cis-acting elements suggested potential associations of AsbZIPs with garlic development, hormone, and stress responses. Several AsbZIPs exhibited tissue-preferential and stress/hormone-responsive expression patterns. Additionally, Asa7G01972 and Asa7G01379 were notably differentially expressed under various stresses and hormone treatments. Subsequent yeast two-hybridization and yeast induction experiments validated their interactions with Asa1G01577, a homologue of ABI5, reinforcing their importance in hormone and abiotic stress responses. This study unveiled the characteristics of the AsbZIP superfamily and lays a solid foundation for further functional analysis of AsbZIP in garlic.
摘要：
■bZIP基因（bZIP）在许多生物过程中是必不可少的，包括发展和应激反应。尽管在许多植物中对BZIP进行了广泛的研究，尚未对大蒜中的bZIP进行全面的全基因组分析。
■在这项研究中，我们鉴定并将64个AsbZIP基因（AsbZIPs）分为10个亚家族。系统分析了这些AsbZIP的进化特征，包括染色体位置，基因结构，保守的图案，和基因复制，进行了。此外，我们还检查了核苷酸多样性，顺式作用元素，和AsbZIPs在各种组织中以及在不同非生物胁迫和激素处理下的表达谱。
■我们的发现表明，基因复制在AsbZIP的扩增中起着至关重要的作用，在驯化过程中观察到轻微的遗传瓶颈。此外，顺式作用元素的鉴定表明AsbZIP与大蒜发育的潜在关联，激素，和应激反应。几个AsbZIP表现出组织偏好和应激/激素响应表达模式。此外,Asa7G01972和Asa7G01379在各种胁迫和激素处理下显著差异表达。随后的酵母两次杂交和酵母诱导实验验证了它们与ABI5的同源物Asa1G01577的相互作用，增强了它们在激素和非生物应激反应中的重要性。本研究揭示了AsbZIP超家族的特征，为进一步分析大蒜中AsbZIP的功能奠定了坚实的基础。
■在这项研究中，我们鉴定并将64个AsbZIP基因（AsbZIPs）分为10个亚家族。系统分析了这些AsbZIP的进化特征，包括染色体位置，基因结构，保守的图案，和基因复制，进行了。此外，我们还检查了核苷酸多样性，顺式作用元素，和AsbZIPs在各种组织中以及在不同非生物胁迫和激素处理下的表达谱。
■我们的发现表明，基因复制在AsbZIP的扩增中起着至关重要的作用，在驯化过程中观察到轻微的遗传瓶颈。此外，顺式作用元素的鉴定表明AsbZIP与大蒜发育的潜在关联，激素，和应激反应。几个AsbZIP表现出组织偏好和应激/激素响应表达模式。此外,Asa7G01972和Asa7G01379在各种胁迫和激素处理下显著差异表达。随后的酵母两次杂交和酵母诱导实验验证了它们与ABI5的同源物Asa1G01577的相互作用，增强了它们在激素和非生物应激反应中的重要性。本研究揭示了AsbZIP超家族的特征，为进一步分析大蒜中AsbZIP的功能奠定了坚实的基础。
