鸡是弯曲杆菌属的主要储库。,主要是空肠杆菌和大肠杆菌,导致人类细菌性胃肠道感染。然而,弯曲杆菌的基因组特征和耐药性。在中低收入国家需要更全面的探索。这项研究旨在使用全基因组测序表征来自商业肉鸡和天然鸡的21个空肠杆菌和5个大肠杆菌分离株,并将它们与28个参考弯曲杆菌序列进行比较。在26个分离株中,在空肠弯曲菌中鉴定了13种序列类型(ST),在大肠杆菌中鉴定了5种ST。突出的ST是ST2274(5个分离株,19.2%),其次是ST51、460、2409和6455(每个ST有2个分离株,7.7%),而所有剩余ST(464、536、595、2083、6736、6964、8096、10437、828、872、900、8237和13540)每个ST有1个分离株(3.8%)。六种类型的抗菌素抗性基因(ant(6)-Ia,aph(3')-III,blaOXA,猫,erm(B),和tet(O)),并检测到gyrA基因(苏氨酸-86-异亮氨酸)和rpsL基因(赖氨酸-43-精氨酸)中的一个点突变。blaOXA抗性基因存在于所有分离物中,gyrA突变在95.2%的空肠弯曲菌和80.0%的大肠杆菌中,76.2%的空肠弯曲菌和80.0%的大肠杆菌中的tet(O)抗性基因。此外,鉴定了与16个毒力因子相关的203个毒力相关基因。在表型抗性方面,空肠弯曲杆菌分离株都对环丙沙星耐药,恩诺沙星,和萘啶酸,对四环素的耐药性较低(76.2%),泰乐菌素(52.3%),红霉素(23.8%),阿奇霉素(22.2%),和庆大霉素(11.1%)。大多数大肠杆菌分离株对所有测试的抗菌药物都有耐药性,而1个大肠杆菌是泛易感的,除了泰乐菌素。单核苷酸多态性的一致性差异很大,与参考弯曲杆菌分离株相比,具有多达13,375个单核苷酸多态性的差异,突出比较基因组之间的遗传差异。这项研究有助于更深入地了解弯曲杆菌属的分子流行病学。在泰国鸡肉生产系统中。
Chickens are the primary reservoirs of Campylobacter spp., mainly C. jejuni and C. coli, that cause human bacterial gastrointestinal infections. However, genomic characteristics and antimicrobial resistance of Campylobacter spp. in low- to middle-income countries need more comprehensive exploration. This study aimed to characterize 21 C. jejuni and 5 C. coli isolates from commercial broilers and native chickens using whole genome sequencing and compare them to 28 reference Campylobacter sequences. Among the 26 isolates, 13 sequence types (ST) were identified in C. jejuni and 5 ST in C. coli. The prominent ST was ST 2274 (5 isolates, 19.2%), followed by ST 51, 460, 2409, and 6455 (2 isolates in each ST, 7.7%), while all remaining ST (464, 536, 595, 2083, 6736, 6964, 8096, 10437, 828, 872, 900, 8237, and 13540) had 1 isolate per ST (3.8%). Six types of antimicrobial resistance genes (ant(6)-Ia, aph(3\')-III, blaOXA, cat, erm(B), and tet(O)) and one point mutations in the gyrA gene (Threonine-86-Isoleucine) and another in the rpsL gene (Lysine-43-Arginine) were detected. The blaOXA resistance gene was present in all isolates, the gyrA mutations was in 95.2% of C. jejuni and 80.0% of C. coli, and the tet(O) resistance gene in 76.2% of C. jejuni and 80.0% of C. coli. Additionally, 203 virulence-associated genes linked to 16 virulence factors were identified. In terms of phenotypic resistance, the C. jejuni isolates were all resistant to ciprofloxacin, enrofloxacin, and nalidixic acid, with lower levels of resistance to tetracycline (76.2%), tylosin (52.3%), erythromycin (23.8%), azithromycin (22.2%), and gentamicin (11.1%). Most C. coli isolates were resistant to all tested antimicrobials, while 1 C. coli was pan-susceptible except for tylosin. Single-nucleotide polymorphisms concordance varied widely, with differences of up to 13,375 single-nucleotide polymorphisms compared to the reference Campylobacter isolates, highlighting genetic divergence among comparative genomes. This study contributes to a deeper understanding of the molecular epidemiology of Campylobacter spp. in Thai chicken production systems.