PDF(Pubmed)
Abstract：
Campylobacter causes bacterial enteritis, dysentery, and growth faltering in children in low- and middle-income countries (LMICs). Campylobacter spp. are fastidious organisms, and their detection often relies on culture independent diagnostic technologies, especially in LMICs. Campylobacter jejuni and Campylobacter coli are most often the infectious agents and in high income settings together account for 95% of Campylobacter infections. Several other Campylobacter species have been detected in LMIC children at an increased prevalence relative to high income settings. After doing extensive whole genome sequencing of isolates of C. jejuni and C. coli in Peru, we observed heterogeneity in the binding sites for the main species-specific PCR assay (cadF) and designed an alternative rpsKD-based qPCR assay to detect both C. jejuni and C. coli. The rpsKD-based qPCR assay identified 23% more C.jejuni/ C.coli samples than the cadF assay among 47 Campylobacter genus positive cadF negative samples verified to have C. jejuni and or C. coli with shotgun metagenomics. This assay can be expected to be useful in diagnostic studies of enteric infectious diseases and be useful in revising the attribution estimates of Campylobacter in LMICs.
摘要：
弯曲杆菌引起细菌性肠炎,痢疾,低收入和中等收入国家（LMICs）儿童的增长步履蹒跚。弯曲杆菌属。是挑剔的生物，它们的检测通常依赖于培养独立的诊断技术，尤其是在LMICs。空肠弯曲杆菌和大肠杆菌弯曲杆菌是最常见的传染因子，在高收入环境中，弯曲杆菌感染占95％。相对于高收入环境，在LMIC儿童中发现了其他几种弯曲杆菌。在对秘鲁的空肠弯曲杆菌和大肠杆菌分离株进行了广泛的全基因组测序后，我们观察到主要物种特异性PCR测定（cadF）的结合位点的异质性，并设计了另一种基于rpsKD的qPCR测定来检测空肠弯曲杆菌和大肠杆菌。在47个弯曲杆菌属阳性cadF阴性样品中，基于rpsKD的qPCR测定鉴定出空肠杆菌/大肠杆菌样品比cadF测定多23％，这些样品被证实具有带有鸟枪宏基因组学的空肠杆菌和/或大肠杆菌。预计该测定法可用于肠道传染病的诊断研究，并可用于修订LMIC中弯曲杆菌的归因估计值。
