这项研究的目的是观察不同水平的硒-壳聚糖,硒的一种新的有机来源,影响了生产性能,鸡蛋质量,鸡蛋硒浓度,微生物种群,免疫反应,抗氧化状态,和蛋黄脂肪酸分布的日本鹌鹑。本实验采用完全随机设计,5种治疗方法6次重复,每次重复10只鸟。饮食处理组如下:不补充硒(对照组),补充0.2mg/kg亚硒酸钠,和0.2、0.4和0.6mg/kg硒-壳聚糖补充剂。与对照组相比,饲喂不同硒壳聚糖水平的鹌鹑的饲料转化率(FCR)线性提高(P<0.05)。此外,浓度为0.2和0.4mg/kg的Se-壳聚糖显示蛋白高度的线性和二次增加,Haugh单位,与对照组相比,新鲜鸡蛋的蛋黄颜色。此外,硒-壳聚糖有助于增强壳的厚度和强度,随着蛋黄中硒浓度的增加。不同水平的硒-壳聚糖补充线性和二次降低了大肠杆菌(COL),同时增加了乳酸菌(LAB)/大肠杆菌的比例(P<0.05)。硒-壳聚糖补充线性和二次增加了对绵羊红细胞(SRBC)和IgG滴度的总抗体反应(P<0.05)。它还线性降低了新鲜和储存的蛋黄中的丙二醛水平,并线性增加了抗氧化酶过氧化氢酶和谷胱甘肽过氧化物酶的活性,鹌鹑血清中超氧化物歧化酶(SOD)呈线性和二次分布(P<0.05)。此外,与对照组相比,以0.2和0.6mg/kg的水平添加硒壳聚糖线性降低了蛋黄中的∑n-6PUFA/∑n-3PUFA比率(P<0.05)。可以得出结论,在蛋鸡的日粮中掺入硒壳聚糖作为硒的新型有机来源,可以提高生产性能。鸡蛋质量,鸡蛋硒浓度,蛋黄脂质氧化,微生物种群,免疫反应,抗氧化酶活性,和蛋黄脂肪酸谱。
The purpose of this research was to see how different levels of Se-chitosan, a novel organic source of Se, affected the production performance, egg quality, egg Se concentration, microbial population, immunological response, antioxidant status, and yolk fatty acid profile of laying Japanese quail. This experiment used a totally randomized design, with 5 treatments, 6 repeats, and 10 birds in each repetition. The dietary treatment groups were as follows: no Se supplementation (control group), 0.2 mg/kg Na-selenite supplementation, and 0.2, 0.4, and 0.6 mg/kg Se-chitosan supplementation. The feed conversion ratio (FCR) improved linearly in quails fed different levels of Se-chitosan compared to the control group (P < 0.05). Furthermore, Se-chitosan at concentrations of 0.2 and 0.4 mg/kg demonstrated both linear and quadratic increases in albumen height, Haugh unit, and yolk color in fresh eggs compared to the control group. Additionally, Se-chitosan contributed to enhanced shell thickness and strength, along with an increased Se concentration in the yolk. Se-chitosan supplementation at different levels linearly and quadratically reduced coliforms (COL) while increasing lactic acid bacteria (LAB)/coliform ratios (P < 0.05). Se-chitosan supplementation linearly and quadratically increased the total
antibody response to sheep red blood cells (SRBC) and IgG titers (P < 0.05). It also linearly decreased the level of malondialdehyde in fresh and stored egg yolks and increased the activity of antioxidant enzymes catalase and glutathione peroxidase linearly, and superoxide dismutase (SOD) both linearly and quadratically in quail blood serum (P < 0.05). Additionally, supplementation of Se-chitosan at levels of 0.2 and 0.6 mg/kg linearly decreased the ∑ n-6 PUFA/∑ n-3 PUFA ratio in the yolk compared to the control group (P < 0.05). It can be concluded that incorporating Se-chitosan as a novel organic source of Se in the diet of laying quails can enhance production performance, egg quality, egg Se concentration, yolk lipid oxidation, microbial population, immune response, antioxidant enzyme activity, and yolk fatty acid profile.