PDF(Pubmed)
Abstract：
The magnitude of the HIV-1 epidemic in Nigeria is second only to the subtype C epidemic in South Africa, yet the subtypes prevalent in Nigeria require further characterization. A panel of 50 subtype G and 18 CRF02_AG Nigerian HIV-1 pseudoviruses (PSV) was developed and envelope coreceptor usage, neutralization sensitivity and cross-clade reactivity were characterized. These PSV were neutralized by some antibodies targeting major neutralizing determinants, but potentially important differences were observed in specific sensitivities (eg. to sCD4, MPER and V2/V3 monoclonal antibodies), as well as in properties such as variable loop lengths, number of potential N-linked glycans and charge, demonstrating distinct antigenic characteristics of CRF02_AG and subtype G. There was preferential neutralization of the matched CRF/subtype when PSV from subtype G or CRF02_AG were tested using pooled plasma. These novel Nigerian PSV will be useful to study HIV-1 CRF- or subtype-specific humoral immune responses for subtype G and CRF02_AG.
摘要：
尼日利亚的HIV-1疫情规模仅次于南非的C亚型疫情,然而,尼日利亚流行的亚型需要进一步表征.开发了一组50个G亚型和18个CRF02_AG尼日利亚HIV-1假病毒（PSV），并使用了包膜共受体，中和敏感性和交叉枝反应性进行了表征。这些PSV被一些针对主要中和决定簇的抗体中和，但是在特定的敏感性上观察到潜在的重要差异（例如。对sCD4，MPER和V2/V3单克隆抗体），以及可变循环长度等属性，潜在的N-连接聚糖和电荷的数量，证明CRF02_AG和G亚型具有不同的抗原特征。当使用混合血浆测试来自G亚型或CRF02_AG的PSV时，匹配的CRF/亚型优先中和。这些新的尼日利亚PSV将用于研究HIV-1CRF-或亚型特异性体液免疫应答的亚型G和CRF02_AG。
