目的:研究尿液代谢组学谱是否可用于鉴定与子宫内膜异位症相关的生物标志物。
方法:前瞻性研究。对于每个主题,在禁食过夜后和手术前收集尿液样本.
方法:大学医学中心。
方法:临床队列包括45例子宫内膜异位症患者,在疾病的早期(n=6)和晚期(n=39)阶段诊断,36位健康女性所有妇女均接受诊断性腹腔镜检查,以目视确认子宫内膜异位病变的存在或不存在。
方法:基于(1)H-核磁共振(NMR)光谱结合统计学方法的尿液样品的代谢组学分析。
方法:比较鉴定子宫内膜异位症患者和健康女性尿液中存在的代谢物。
结果:子宫内膜异位症患者的尿液代谢组学表现出更高浓度的N(1)-甲基-4-吡啶酮-5-甲酰胺,胍基琥珀酸酯,肌酐,牛磺酸,缬氨酸,与健康女性相比,2-羟基异戊酸和赖氨酸浓度降低。这些代谢物中的大多数参与炎症和氧化应激过程。这些病理生理事件先前被描述为存在于异位子宫内膜增殖灶中。
结论:总体而言,结果证明了(1)基于H-NMR的代谢组学的潜力,一种快速、无创的方法,确定尿液样本中与子宫内膜异位症相关的代谢变化。这些信息可能有助于更好地了解子宫内膜异位症的发病机制,从而为这种病理的非侵入性诊断提供支持。
OBJECTIVE: To investigate whether urine metabolomic profile can be used to identify biomarkers associated to endometriosis.
METHODS: Prospective study. For each subject, a urine sample was collected after overnight fasting and before surgery.
METHODS: University medical center.
METHODS: The clinical cohort included 45 endometriosis patients, diagnosed at early (n = 6) and advanced (n = 39) stages of the disease, and 36 healthy women. All women underwent diagnostic laparoscopy to visually confirm the presence or absence of endometriotic lesions.
METHODS: Metabolomic profiling of urine samples based on (1)H-nuclear magnetic resonance (NMR) spectroscopy in combination with statistical approaches.
METHODS: Comparative identification of metabolites present in urine from endometriosis patients and healthy women.
RESULTS: The urine metabolomic profile of endometriosis patients exhibited higher concentrations of N(1)-methyl-4-pyridone-5-carboxamide, guanidinosuccinate, creatinine, taurine, valine, and 2-hydroxyisovalerate and decreased concentrations of lysine compared with healthy women. Most of these metabolites are involved in inflammation and oxidative stress processes. These pathophysiologic events had been previously described to be present in ectopic endometrial proliferation foci.
CONCLUSIONS: Overall, the results demonstrate the potential of (1)H-NMR-based metabolomics, a rapid and noninvasive approach, to identify metabolic changes associated to endometriosis in urine samples. This information could be useful to get a better understanding of the pathogenesis of endometriosis, thus providing support to the noninvasive diagnosis of this pathology.