炎症和免疫失调可能与精神分裂症的发病有关。骨桥蛋白(OPN)是一种细胞因子样糖蛋白,参与炎症和调节免疫反应,它还可以直接修饰小胶质细胞的细胞因子表达和存活。此外,最近已经描述了在首发精神病中OPN的基因表达升高,但迄今为止,OPN水平尚未在精神分裂症中进行研究。T辅助亚型的失衡也可能代表精神分裂症的脆弱性因素。在这项研究中,我们分析了OPN的浓度,22例精神分裂症患者中与T辅助亚型相关的细胞因子水平:Th1的干扰素γ(IFNy),Th2的白介素(IL)-10,Th17的IL-8和中性粒细胞与淋巴细胞比率(NLR)通过阳性和阴性综合征量表(PANSS)和临床总体印象量表(CGI)评分评估其症状强度。血清OPN,IFNY,通过ELISA试剂盒测量IL-10和IL-8浓度,并从血细胞计数计算NLR。我们发现OPN水平与PANSS总分和PANSS总分之间存在显着相关性。IFNy水平和NLR与PANSS总水平显著相关,PANSS阳性,PANSS-一般,和CGI得分。在测量的标志物中,抗精神病药物治疗仅对NLR和OPN水平有显著影响,长期抗精神病药物治疗后两者均显著降低.我们的结果表明,升高的OPN和IFNy浓度,和NLR升高与精神分裂症的严重症状相关,提示Th1亚型在PANSS阳性和PANSS-一般亚评分高的患者中的重要性。NLR和PANSS评分之间的显著相关性加强了精神分裂症的炎症假说。
Inflammation and immune dysregulation could contribute to the pathogenesis of schizophrenia. Osteopontin (OPN) is a cytokine-like glycoprotein involved in inflammation and in modulating immune responses, and it can also directly modify the cytokine expression and survival of microglia. Furthermore, elevated gene expression of OPN in first episode psychosis has recently been described, but to date OPN level has not been investigated in schizophrenia. Imbalance of T-helper subtypes could also represent a vulnerability factor for schizophrenia. In this study, we analyzed the concentration of OPN, levels of cytokines associated with T-helper subtypes: interferon gamma (IFNy) for Th1, interleukin (IL)-10 for Th2, IL-8 for Th17, and neutrophil-to-lymphocyte ratio (NLR) in 22 patients with schizophrenia assessed for the intensity of their symptoms by the Positive and Negative Syndrome Scale (PANSS) and Clinical Global Impression scale (CGI) scores. Serum OPN, IFNy, IL-10, and IL-8 concentrations were measured by ELISA kits and NLR was calculated from blood count. We found significant correlation between the level of OPN and PANSS-total and PANSS-general scores. IFNy level and NLR showed significant correlation with PANSS-total, PANSS-positive, PANSS-general, and CGI score. Among the measured markers antipsychotic therapy only had significant effects on NLR and OPN level, both of which were significantly reduced after long-term antipsychotic treatment. Our results indicate that elevated OPN and IFNy concentrations, and increased NLR are associated with severe symptoms in schizophrenia and suggest the importance of Th1 subtype in patients with high PANSS-positive and PANSS-general subscore. Significant correlation between NLR and PANSS scores strengthens the inflammation hypothesis of schizophrenia.