■敏感头皮是与敏感皮肤综合症有关的最常见的疾病之一,在没有可见的炎症迹象的情况下表现为令人不快的感觉反应。在这项研究中,研究了在分类学水平上局部应用喜马拉雅来源的酵母菌和乳酸菌发酵复合物(SLFC)对细菌和真菌头皮微生物组的影响以及敏感性皮肤综合征的缓解。
■首先,根据问卷将健康女性参与者(30~45岁)分为健康头皮组和敏感头皮组.此后,评估了SLFC在敏感头皮和头皮微生物组上的局部应用,使用16SrRNA和ITS1测序分析评估健康和敏感头皮群落之间微生物分类群的分布差异。此外,SLFC在粉刺杆菌的物种水平上对头皮微生物组的影响,表皮葡萄球菌,和马拉色菌限制性酶通过qPCR评估。
■用SLFC治疗28天后,葡萄球菌的丰度,Lawsonella,敏感头皮组镰刀菌高度显着增加(p<0.001),而镰刀菌和马拉色菌的丰度显著降低(p<0.001)。此外,自我评估问卷显示,每天两次应用SLFC,28天后,综合征缓解效果为100%.
■获得的结果将有助于更好地了解敏感头皮的微生物群落,并提供关于利用SLFC来维持健康头皮和调节头皮微生物组的有用信息。
UNASSIGNED: Sensitive scalp is one of the most frequent complaints related to sensitive skin syndrome, characterized by unpleasant sensory reactions in the absence of visible signs of inflammation. In this study, the effects of topical application of postbiotic Himalaya-derived Saccharomyces and Lactobacillus ferment complex (SLFC) on the bacterial and fungal scalp microbiome at the taxonomic level and alleviation of sensitive skin syndrome were investigated.
UNASSIGNED: Firstly, healthy female participants (aged 30-45) were classified into a healthy scalp group and a sensitive scalp group based on the questionnaire. Thereafter, topical application of SLFC on sensitive scalp as well as scalp microbiome was evaluated, with the difference in the distribution of microbial taxa between healthy and sensitive scalp communities was assessed using 16S rRNA and ITS1 sequencing analysis. In addition, the effect of SLFC on scalp microbiome at the species level for Cutibacterium acnes, Staphylococcus epidermidis, and Malassezia restricta was evaluated by the qPCR assessment.
UNASSIGNED: After treatment with SLFC for 28 days, the abundance of Staphylococcus, Lawsonella, and Fusarium in the sensitive scalp group was highly significantly increased (p < 0.001), while the abundance of Cutibacterium and Malassezia was highly significantly decreased (p < 0.001). Furthermore, the self-assessment questionnaire indicated a syndrome alleviation effect of 100% after 28 days with a twice-daily application of the SLFC.
UNASSIGNED: The obtained results would help to better understand the microbial community of the sensitive scalp and provide useful information on utilization of SLFC for maintaining a healthy scalp and modulating the scalp microbiome.