背景:这项研究的目的是分析血清PYCARD(含Pyrin和CARD结构域的蛋白质,通常称为ASC-凋亡相关斑点样蛋白,含有caspase激活和募集结构域),白细胞介素-38(IL-38),和白细胞介素-6(IL-6)在患有类风湿性关节炎(RA)的个体中。
方法:我们的研究包括2021年11月至2023年6月期间在承德医科大学附属医院就诊的88例RA患者,构成试验组。此外,对照组为88名在上述时间范围内在同一医院接受健康评估的患者,作为对比.该研究涉及IL-38,IL-6,PYCARD,抗环瓜氨酸肽抗体(抗CCP),和红细胞沉降率(ESR)水平两组。本研究旨在探讨这些标志物的相关性和诊断效能,采用相关的统计分析进行综合评价。
结果:试验组PYCARD的表达水平较高,IL-6、IL-38优于对照组(P<0.05)。基于相关性分析,PYCARD与IL-38有较强的相关性(P<0.01)。当使用PYCARD和抗CCP的组合时,受试者工作特征(ROC)曲线分析显示曲线下面积(AUC)值为0.97、0.96和0.96,IL-38和抗CCP,IL-6和抗CCP预测RA,分别。重要的是,与PYCARD相比,这三对都表现出优异的AUC值,IL-38,IL-6,ESR,或用作独立诊断指标的抗CCP。
结论:PYCARD,IL-6和IL-38作为新的诊断标志物显示出有希望的潜力,并且可能构成支持RA诊断的有价值的工具。
BACKGROUND: The objective of this investigation is to analyze the levels and clinical relevance of serum PYCARD (Pyrin and CARD domain-containing protein, commonly known as ASC-apoptosis-associated speck-like protein containing a caspase activation and recruitment domain), interleukin-38 (IL-38), and interleukin-6 (IL-6) in individuals afflicted with rheumatoid arthritis (RA).
METHODS: Our study comprised 88 individuals diagnosed with RA who sought medical attention at the Affiliated Hospital of Chengde Medical University during the period spanning November 2021 to June 2023, constituting the test group. Additionally, a control group of 88 individuals who underwent health assessments at the same hospital during the aforementioned timeframe was included for comparative purposes. The study involved the assessment of IL-38, IL-6, PYCARD, anti-cyclic citrullinated peptide antibody (anti-CCP), and erythrocyte sedimentation rate (ESR) levels in both groups. The research aimed to explore the correlations and diagnostic efficacy of these markers, employing pertinent statistical analyses for comprehensive evaluation.
RESULTS: The test group had higher expression levels of PYCARD, IL-6, and IL-38 than the control group (P < 0.05). Based on the correlation analysis, there was a strong relationship between PYCARD and IL-38 (P < 0.01). The receiver operating characteristic (ROC) curve analysis revealed area under the curve (AUC) values of 0.97, 0.96, and 0.96 when using combinations of PYCARD and anti-CCP, IL-38 and anti-CCP, and IL-6 and anti-CCP for predicting RA, respectively. Importantly, all three of these pairs demonstrated superior AUC values compared to PYCARD, IL-38, IL-6, ESR, or anti-CCP used as standalone diagnostic indicators.
CONCLUSIONS: PYCARD, IL-6, and IL-38 exhibit promising potential as novel diagnostic markers and may constitute valuable tools for supporting the diagnosis of RA.