背景：在过去的30年中，头颈部鳞状细胞癌（HNSCC）的总死亡率没有改善，主要是因为区域转移性疾病患者的高治疗失败率。为了更好地了解导致淋巴转移发展的病理生物学过程，迫切需要相关的动物模型。
方法:将HNSCC细胞系植入无胸腺裸鼠的舌头中。组织学,免疫组织化学，和离体双光子显微镜用于评估肿瘤的进展和扩散。
结果：不同HNSCC细胞系的原位异种移植物产生了不同的存活模式，肿瘤组织学,疾病进展率,和淋巴结转移的发展。值得注意的是,所有注射的细胞类型在注射后24小时内到达淋巴结，但不是所有的都有转移.
结论：这种原位异种移植模型紧密地模拟了人类癌症的几个特征，对于关注淋巴转移发展和病理学的转化研究可能非常有价值。
BACKGROUND: The overall mortality rate in cases of head and neck squamous cell carcinoma (HNSCC) has not improved over the past 30 years, mostly because of the high treatment failure rate among patients with regionally metastatic disease. To better understand the pathobiologic processes leading to lymphatic metastasis development, there is an urgent need for relevant animal models.
METHODS: HNSCC cell lines were implanted into the tongues of athymic nude mice. Histology, immunohistochemistry, and ex vivo 2-photon microscopy were used to evaluate tumor progress and spread.
RESULTS: Orthotopic xenografts of different HNSCC cell lines produced distinct patterns of survival, tumor histology, disease progression rate, and lymph node metastasis development. Remarkably, all injected cell types reached the lymph nodes within 24 hours after injection, but not all developed metastasis.
CONCLUSIONS: This orthotopic xenograft model closely mimics several characteristics of human cancer and could be extremely valuable for translational studies focusing on lymphatic metastasis development and pathobiology.
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