背景:在胎儿羔羊的肾小球形成过程中产生梗阻性尿路病变(OU)导致足月肾脏多囊性发育不良(MCDK)。我们使用免疫组织化学技术探索了这一点。
方法:在妊娠60天的胎儿羔羊中创建了OU,结扎尿道和脐。MCDK羔羊的肾脏,妊娠60天的胎儿羔羊,足月羔羊(145天),假手术羔羊,并对成年母羊进行HE染色,并用配对盒基因2(PAX2)和CD10进行免疫组织化学。
结果:在MCDK模型中发现多个囊肿。CD10在近端肾小管上皮细胞中表达,肾小球上皮细胞,妊娠60天胎羔羊、足月羔羊和成年母羊肾脏中的髓质基质细胞。在输尿管芽中发现PAX2表达,C形和S形的身体,收集管的上皮细胞,和Bowman在妊娠60天的胎儿肾脏胶囊,但只在足月羔羊和成年母羊的集合管中。CD10和PAX2均在MCDK模型的囊性上皮细胞中表达。
结论:囊性上皮细胞中PAX2的表达表明,在OU模型的肾脏发育过程中,囊肿的形成与肾原区上皮细胞中PAX2的下调紊乱有关。
BACKGROUND: Creating obstructive uropathy (OU) during glomerulogenesis in the fetal lamb results in multicystic dysplastic kidney (MCDK) at term. We explored this using immunohistochemical techniques.
METHODS: OU was created in fetal lambs at 60-day gestation, ligating the urethra and urachus. The kidneys of MCDK lambs, 60-day gestation fetal lambs, full-term lamb (145 days), term sham-operated lambs, and adult ewes were evaluated by HE staining, and immunohistochemistry with paired box genes 2 (
PAX2) and CD10.
RESULTS: Multiple cysts were found in the MCDK model. CD10 was expressed in proximal tubular epithelial cells, glomerular epithelial cells, and medullary stromal cells in the kidneys of 60-day gestation fetal lambs and full-term lambs and adult ewes.
PAX2 expression was found in ureteric buds, C- and S-shaped bodies, epithelial cells of collecting ducts, and Bowman\'s capsule of fetal kidneys at 60-day gestation, but only in the collecting ducts of full-term fetal lambs and adult ewes. Both CD10 and
PAX2 were expressed in the cystic epithelial cells of the MCDK model.
CONCLUSIONS: PAX2 expression in cystic epithelial cells suggests that cyst formation is associated with disturbed down-regulation of
PAX2 in the nephrogenic zone epithelial cells during the renal development in the OU model.