背景:在我们的工厂,以前使用20号标准针进行卵母细胞提取,该标准针整体均匀薄(tSN);但是最近,我们开始使用减少的针头,20号针尖和17号针体(RN)。直到现在,有RN和粗标准针之间的比较,但是RN和tSN之间没有比较。这项研究的目的是比较使用RN和tSN的卵母细胞检索结果。
方法:从2020年1月至2023年12月在我们的机构进行的304个周期的医疗记录中提取了有关卵母细胞检索的信息。回顾性比较了两种类型针头的卵母细胞取出结果,抗苗勒管激素(AMH),程序时间,额外的镇静剂,刺破的卵泡数量,回收的卵母细胞数量,受精的卵母细胞数量,卵母细胞回收率,和施肥率。
结果:当AMH≥1.2ng/mL时,RN和tSN组的手术时间分别为9.3±3.7和12.1±4.6分钟,分别为(P<0.001),对其他镇静剂的需求也有显著差异:RN组为54.0%,tSN组为78.5%(P=0.002).RN组和tSN组的卵母细胞回收率分别为65.3%和61.2%,差异有显著性,分别为(P=0.046),RN和tSN组之间的受精率差异显着,分别为56.8%和66.8%,分别(P<0.001)。年龄没有显着差异,AMH,刺破的卵泡数量,回收的卵母细胞数量,或受精的卵母细胞数量。
结论:卵巢储备不减少,与tSN相比,RN减少了手术时间,并且需要额外的镇静剂。此外,每次取卵受精的卵母细胞数量保持不变,表明卵母细胞回收性能不受影响。
BACKGROUND: At our facility, oocyte retrieval had previously been performed with a 20-gauge standard needle that is uniformly thin overall (tSN); but recently, we have instead started using reduced needles, with a 20-gauge tip and 17-gauge body (RN). Until now, there have been comparisons between RN and thick standard needles, but there have been no comparisons between RN and tSN. The purpose of this study was to compare oocyte retrieval outcomes using RN with tSN.
METHODS: Information on oocyte retrieval was extracted from the medical records of 304 cycles performed at our facility from January 2020 to December 2023. The oocyte retrieval outcomes of the two types of needles were compared retrospectively with respect to age, anti-Müllerian hormone (AMH), procedure time, additional sedatives, number of follicles punctured, number of oocytes retrieved, number of oocytes fertilized, oocyte recovery rate, and fertilization rate.
RESULTS: When AMH ≥ 1.2 ng/mL, the procedure time was 9.3 ± 3.7 and 12.1 ± 4.6 minutes in the RN and tSN groups, respectively (P < 0.001), and the need for additional sedatives was also significantly different: 54.0% in the RN group and 78.5% in the tSN group (P = 0.002). The oocyte recovery rate was significantly different between the RN and tSN groups at 65.3% and 61.2%, respectively (P = 0.046), and the fertilization rate was significantly different between the RN and tSN groups at 56.8% and 66.8%, respectively (P < 0.001). There were no significant differences by age, AMH, number of follicles punctured, number of oocytes retrieved, or number of oocytes fertilized.
CONCLUSIONS: Without diminished ovarian reserve, RN reduced procedure time and the need for additional sedatives compared to tSN. In addition, the number of oocytes fertilized per oocyte retrieval remained the same, indicating that oocyte retrieval performance was not affected.