硫化焦菌是土壤中普遍存在的铁还原细菌,在工程系统中,它可以呼吸电极以产生可测量的电流。它独特的新陈代谢,严重依赖广泛的细胞色素网络,需要独特的细胞组成。在这项工作中,我们使用了金属组学,细胞分数和元素分析,和转录组学来研究和分析硫还原G的细胞组成。元素组成研究(C,H,O,N,和灰分含量)显示出约1.7:1和0.25:1的高C:O和H:O比率,表明与高脂质含量一致的更多减少的细胞组成。我们的研究表明,硫还原G。细胞具有大量的铁(2±0.2μg/g干重)和脂质(32±0.5%干重/干重),并且该组成不会改变细胞是否与可溶性或不溶性电子受体一起生长。高的铁浓度,高于类似的微生物,归因于在固体和可溶性电子受体生长的转录组学分析中丰富的细胞色素的产生。在培养这种微生物进行实验室研究和商业应用时,必须考虑硫还原G.的独特细胞组成。重要性硫化还原焦菌是一种电活性微生物。在大自然中,它通过将电子转移到金属矿物上而生长,有效地“呼吸”金属。在人造系统中,它使电极产生电流。它已成为研究电活性生物的模式生物。生物的潜在生物技术应用可以弥合生物学和电信号之间的差距,作为世界各地土壤中普遍存在的铁减速器,G.硫还原对全球铁循环有影响。我们测量了金属的浓度,大分子,和G中的基本元素硫还原来定义这种生物的组成。我们还使用基因表达数据来讨论这些金属可能与哪些蛋白质相关。我们发现,与其他细菌相比,硫还原菌具有大量的脂质和铁-这些观察结果对于未来与生物体合作的微生物学家和生物技术学家很重要。
Geobacter sulfurreducens is a ubiquitous iron-reducing bacterium in soils, and in engineered systems, it can respire an electrode to produce measurable electric current. Its unique metabolism, heavily dependent on an extensive network of cytochromes, requires a unique cell composition. In this work, we used metallomics, cell fraction and elemental analyses, and transcriptomics to study and analyze the cell composition of G. sulfurreducens. Elemental composition studies (C, H, O, N, and ash content) showed high C:O and H:O ratios of approximately 1.7:1 and 0.25:1, indicative of more reduced cell composition that is consistent with high lipid content. Our study shows that G. sulfurreducens cells have a large amount of iron (2 ± 0.2 μg/g dry weight) and lipids (32 ± 0.5% dry weight/dry weight) and that this composition does not change whether the cells are grown with a soluble or an insoluble electron acceptor. The high iron concentration, higher than similar microorganisms, is attributed to the production of cytochromes that are abundant in transcriptomic analyses in both solid and soluble electron acceptor growth. The unique cell composition of G. sulfurreducens must be considered when growing this microorganism for lab studies and commercial applications. IMPORTANCE Geobacter sulfurreducens is an electroactive microorganism. In nature, it grows on metallic minerals by transferring electrons to them, effectively \"breathing\" metals. In a manmade system, it respires an electrode to produce an electric current. It has become a model organism for the study of electroactive organisms. There are potential biotechnological applications of an organism that can bridge the gap between biology and electrical signal and, as a ubiquitous iron reducer in soils around the world, G. sulfurreducens has an impact on the global iron cycle. We measured the concentrations of metals, macromolecules, and basic elements in G. sulfurreducens to define this organism\'s composition. We also used gene expression data to discuss which proteins those metals could be associated with. We found that G. sulfurreducens has a large amount of lipid and iron compared to other bacteria-these observations are important for future microbiologists and biotechnologists working with the organism.