线粒体是Fe-S簇合成的关键细胞器。它们含有半胱氨酸脱硫酶,一种支架蛋白,铁和电子供体,和特定的分子伴侣都是形成Fe-S团簇所必需的。新形成的簇可以通过线粒体Fe-S蛋白合成来利用或进行进一步的转化。线粒体Fe-S簇生物生成成分是胞质铁硫簇组装机制中需要的,用于胞质和核簇供应。作为Fe-S蛋白的关键组分的簇在暴露于氧化应激时是脆弱的并且易于降解。然而,一旦退化,Fe-S团簇可以重新合成或修复。有人提出硫转移酶,罗丹,和3-巯基丙酮酸硫转移酶,负责硫从供体转移到亲核受体,参与了Fe-S团簇的形成,成熟,或重建。在本论文中,我们试图总结我们对硫转移酶不仅在硫管理中而且在哺乳动物和酵母中Fe-S簇形成中的参与的知识,以及重建受损的簇或恢复酶减弱的活性。
Mitochondria are the key organelles of Fe-S cluster synthesis. They contain the enzyme cysteine desulfurase, a scaffold protein, iron and electron donors, and specific chaperons all required for the formation of Fe-S clusters. The newly formed cluster can be utilized by mitochondrial Fe-S protein synthesis or undergo further transformation. Mitochondrial Fe-S cluster biogenesis components are required in the cytosolic iron-sulfur cluster assembly machinery for cytosolic and nuclear cluster supplies. Clusters that are the key components of Fe-S proteins are vulnerable and prone to degradation whenever exposed to oxidative stress. However, once degraded, the Fe-S cluster can be resynthesized or repaired. It has been proposed that sulfurtransferases, rhodanese, and 3-mercaptopyruvate sulfurtransferase, responsible for sulfur transfer from donor to nucleophilic acceptor, are involved in the Fe-S cluster formation, maturation, or reconstitution. In the present paper, we attempt to sum up our knowledge on the involvement of sulfurtransferases not only in sulfur administration but also in the Fe-S cluster formation in mammals and yeasts, and on reconstitution-damaged cluster or restoration of enzyme\'s attenuated activity.