hanganumharmalaL.(P.harmala),也被称为Espand,哈梅尔,或者叙利亚的Rue,和贯叶连翘(H.perforatum),通常被称为圣约翰草,是两种广泛种植的工业作物,在全球范围内用于抗肝癌相关产品。然而,它们的主要功能物质仍然不清楚,从而阻碍了世界各地相关产品的疗效评价和质量控制。在这项工作中,通过建立主成分分析(PCA)-HPLC次级代谢产物作图模型,阐明了P.harmala和H.perforatum的抗肝癌生物标志物.通过HPLC对植物提取物的化学指纹图谱进行分析,然后使用PCA绘制以产生次级代谢产物模型。这些模型将化学信息与植物提取物的抗肝癌活性相关联,从而表明P.harmala和H.perforatum抗肝癌细胞的功能抑制剂。所鉴定的化合物的活性通过细胞毒性和凋亡测定来验证。确定了P.harmala和H.perforatus对人肝癌的主要抑制剂是harmine和槲皮素,分别。harmine对HepG2细胞的IC50值和诱导凋亡率分别为20.7±2.8μM和46.7±3.5%。分别。槲皮素对HepG2细胞的IC50值和诱导凋亡率分别为49.5±6.6μM和38.7±2.6%。分别。总之,结果大大扩展了对P.harmala和H.perforatum的生化基础的理解,因此,显然支持他们目前在世界各地的应用。此外,harmine和槲皮素可作为生物标志物,用于评估工业作物相关产品在治疗和改善健康应用中的功效和质量。
Peganum harmala L. (P. harmala), also known as Espand, Harmel, or Syrian rue, and Hypericum perforatum L. (H. perforatum), commonly known as St. John\'s wort, are two of the widely cultivated industrial crops and used worldwide in antihepatoma-related products. However, their main functional substances are still not clear, thus impeding the efficacy evaluations and quality controls of relative products around the world. In this work, the anti-
hepatoma biomarkers of P. harmala and H. perforatum were clarified through the development of principal components analysis (PCA)-HPLC secondary metabolite mapping models. The chemical fingerprints of plant extracts were profiled by HPLC and then mapped to produce the secondary metabolite models using PCA. The models correlated the chemical information with the anti-
hepatoma activities of plant extracts, thus indicating the functional inhibitors of P. harmala and H. perforatum against
hepatoma cells. The activities of the identified compounds were validated by cytotoxic and apoptotic assays. The major inhibitors of P. harmala and H. perforatum against human
hepatoma were determined to be harmine and quercetin, respectively. The IC50 values and the induced apoptotic rate of harmine on HepG2 cells were 20.7 ± 2.8 μM and 46.7 ± 3.5 %, respectively. The IC50 values and the induced apoptotic rate of quercetin on HepG2 cells were 49.5 ± 6.6 μM and 38.7 ± 2.6 %, respectively. In conclusion, the results significantly expanded the understanding of the biochemical foundations of P. harmala and H. perforatum, thus evidently supporting their current applications around the world. Moreover, harmine and quercetin could be used as biomarkers to evaluate the efficacy and quality of related products of industrial crops in therapeutic and health-improving applications.