背景:嗜酸性粒细胞性胃炎(EoG)与2型(T2)免疫相关。然而,T2细胞因子细胞来源,胃T细胞组成,胃T细胞与疾病病理的关系仍未得到充分研究。
目的:我们定义了胃T细胞群及其与组织学和内镜下EoG病理的关系。
方法:胃活检(n=6EoG,n=7对照)接受组织学检查,内窥镜,和流式细胞术分析。在互补队列中(n=83EoG),IL4、IL5和IL13mRNA水平与EoG病理参数相关。
结果:胃活检包含主要为CD8+的CD3+T细胞;CD8/CD4比率在EoG和对照活检中相当(分别为4.0±2.0和4.3±1.1;P=0.76)。胃T调节(Treg,与对照组相比,EoG中的CD3+CD4+FOXP3+)和T辅助型2(Th2,CD3+CD4+GATA3+)细胞水平增加(2倍,P<0.05和10倍,分别为P<0.001)并与胃嗜酸性粒细胞水平相关(分别为r=0.63,P<0.05和r=0.85,P<0.001),内镜病理(r=0.56,P<0.01;r=0.84,P<0.001;分别)和组织病理学(r=0.72,P<0.01;r=0.82,P<0.01;分别)。细胞因子阳性,最值得注意的是IL-4+,Th2细胞水平与组织学和内镜评分密切相关(分别为r=0.89,P<0.0001和r=0.78,P<0.0001)。在一个独立的EoG队列中(n=83),大量胃IL4,IL5和IL13mRNA水平与组织学评分(分别为r=0.22,P<0.005;r=0.54,P<0.0001;r=0.36,P<0.0001)和内镜评分(分别为r=0.27,P<0.001;r=0.40,P<0.0001;r=0.35,P<0.0001)相关。
结论:EoG是一种Th2细胞相关疾病,其特征是胃T2细胞因子产生的CD3+CD4+GATA3+Th2细胞增加,与疾病病理密切相关。
Eosinophilic gastritis (EoG) associates with type 2 immunity. However, the type 2 cytokine cellular source, gastric T-cell composition, and gastric T-cell relationship (or relationships) with disease pathology remain understudied.
We defined gastric T-cell populations and their association with histologic and endoscopic EoG pathology.
Gastric biopsy samples (n = 6 EoG, n = 7 control) were subjected to histologic, endoscopic, and flow cytometry analyses. In a complementary cohort (n = 83 EoG), IL4, IL5, and IL13 mRNA levels were correlated with EoG pathologic parameters.
Gastric biopsy samples contained CD3+ T cells that were mainly CD8+; the CD8/CD4 ratio was comparable in EoG and control biopsy samples (5.7 ± 3.0 and 4.3 ± 0.6, respectively; P = .28). Gastric regulatory T (CD3+CD4+FOXP3+) and TH2 (CD3+CD4+GATA3+) cell levels were increased in EoG versus controls (2-fold, P < .05 and 10-fold, P < .001, respectively) and correlated with gastric eosinophil levels (r = 0.63, P < .05 and r = 0.85, P < .001, respectively), endoscopic pathology (r = 0.56, P < .01; r = 0.84, P < .001, respectively), and histopathology (r = 0.72, P < .01; r = 0.82, P < .01, respectively). Cytokine-positive, most notably IL-4+, TH2 cell levels strongly correlated with histologic and endoscopic scores (r = 0.82, P < .0001 and r = 0.78, P < .0001, respectively). In an independent EoG cohort (n = 83), bulk gastric IL4, IL5, and IL13 mRNA levels correlated with histologic score (r = 0.22, P < .005; r = 0.54, P < .0001; and r = 0.36, P < .0001, respectively) and endoscopic score (r = 0.27, P < .001; r = 0.40, P < .0001; and r = 0.35, P < .0001, respectively).
EoG is a TH2 cell-associated disease featuring increased gastric type 2 cytokine-producing CD3+CD4+GATA3+TH2 cells that strongly correlate with disease pathologies.