背景:上呼吸道粘膜在呼吸道的物理完整性和免疫功能中起着至关重要的作用。然而,在某些情况下,如感染,创伤,或者手术,它可能会受到损害。组织工程,再生医学领域,已在各种医疗领域找到应用,包括但不限于整形手术,眼科,还有泌尿科.然而,由于上呼吸道的复杂形态和组织学,其在呼吸系统的应用仍然有些困难。迄今为止,一种生产可处理的文化协议,分化良好的鼻粘膜尚未发育。这篇综述的目的是描述与用于生产自体健康人类上呼吸道细胞和粘膜组织的细胞培养技术有关的研究现状,以及描述其临床应用。
方法:在整个Embase没有时间限制的情况下,进行了相关文献的搜索,科克伦,PubMed,和MedlineOvid数据库。与“呼吸道粘膜”和“人气道培养技术”相关的关键词是本综述的搜索策略的重点。使用JoannaBriggs研究所(JBI)的定性研究关键评估工具评估保留研究中的偏倚风险。然后对我们的结果进行了叙述性综合。
结果:本综述共纳入33项研究,其中13个只专注于开发细胞培养方案,而无需进一步使用。其余的研究使用自己开发的协议,用于各种应用,如囊性纤维化,药理学,和病毒研究。一项研究能够开发出一种有前途的鼻粘膜模型,可用作鼻气管重建手术的替代品。
结论:本系统综述广泛地探讨了有关生产组织工程鼻粘膜的细胞培养技术的研究现状。鼻粘膜的生物工程具有巨大的临床应用潜力。然而,对机械性能的进一步研究至关重要,因为工程组织的比较目前侧重于形态学而不是全面的力学评估。
BACKGROUND: The upper respiratory mucosa plays a crucial role in both the physical integrity and immunological function of the respiratory tract. However, in certain situations such as infections, trauma, or surgery, it might sustain damage. Tissue engineering, a field of regenerative medicine, has found applications in various medical fields including but not limited to plastic surgery, ophthalmology, and urology. However, its application to the respiratory system remains somewhat difficult due to the complex morphology and histology of the upper respiratory tract. To date, a culture protocol for producing a handleable, well-differentiated nasal mucosa has yet to be developed. The objective of this review is to describe the current state of research pertaining to cell culture techniques used for producing autologous healthy human upper respiratory cells and mucosal tissues, as well as describe its clinical applications.
METHODS: A search of the relevant literature was carried out with no time restriction across Embase, Cochrane, PubMed, and Medline Ovid databases. Keywords related to \"respiratory mucosa\" and \"culture techniques of the human airway\" were the focus of the search strategy for this review. The risk of bias in retained studies was assessed using the Joanna Briggs Institute\'s (JBI) critical appraisal tools for qualitative research. A narrative synthesis of our results was then conducted.
RESULTS: A total of 33 studies were included in this review, and thirteen of these focused solely on developing a cell culture protocol without further use. The rest of the studies used their own developed protocol for various applications such as cystic fibrosis, pharmacological, and viral research. One study was able to develop a promising model for nasal mucosa that could be employed as a replacement in nasotracheal reconstructive surgery.
CONCLUSIONS: This systematic review extensively explored the current state of research regarding cell culture techniques for producing tissue-engineered nasal mucosa. Bioengineering the nasal mucosa holds great potential for clinical use. However, further research on mechanical properties is essential, as the comparison of engineered tissues is currently focused on morphology rather than comprehensive mechanical assessments.