在需要重症监护的重症患者中,氧化应激的增加在发病机制中起着重要作用。镇静剂广泛用于许多这些患者的镇静。一些镇静剂是已知的抗氧化剂。然而,没有研究评估各种镇静剂对不同自由基的直接清除活性。本研究旨在确定常见的镇静剂(异丙酚,硫喷妥钠,和右美托咪定(DEX))具有使用体外电子自旋共振对各种自由基的直接清除活性。超氧化物,羟基自由基,单线态氧,并测定了一氧化氮(NO)的直接清除活性。所有镇静剂清除不同类型的自由基。DEX,一种新的镇静剂,还清除了羟基自由基。硫喷妥钠清除所有类型的自由基,包括NO,而异丙酚不能清除超氧自由基。在这个回顾性分析中,我们观察到重度颅脑外伤患者服用硫喷妥钠后氧化抗氧化剂标志物的变化.我们确定了临床中使用的各种镇静剂的直接自由基清除活性。此外,我们报道了一例具有代表性的创伤性脑损伤病例,其中硫喷妥钠显著影响氧化应激相关生物标志物.这项研究表明,在未来,含硫喷妥钠的镇静剂可以通过进一步的临床研究重新开发为抗氧化疗法。
In critically ill patients requiring intensive care, increased oxidative stress plays an important role in pathogenesis. Sedatives are widely used for sedation in many of these patients. Some sedatives are known antioxidants. However, no studies have evaluated the direct scavenging activity of various sedative agents on different free radicals. This study aimed to determine whether common sedatives (propofol, thiopental, and dexmedetomidine (DEX)) have direct free radical scavenging activity against various free radicals using in vitro electron spin resonance. Superoxide, hydroxyl radical, singlet oxygen, and nitric oxide (NO) direct scavenging activities were measured. All sedatives scavenged different types of free radicals. DEX, a new sedative, also scavenged hydroxyl radicals. Thiopental scavenged all types of free radicals, including NO, whereas propofol did not scavenge superoxide radicals. In this retrospective analysis, we observed changes in oxidative antioxidant markers following the administration of thiopental in patients with severe head trauma. We identified the direct radical-scavenging activity of various sedatives used in clinical settings. Furthermore, we reported a representative case of traumatic brain injury wherein thiopental administration dramatically affected oxidative-stress-related biomarkers. This study suggests that, in the future, sedatives containing thiopental may be redeveloped as an antioxidant therapy through further clinical research.