具有多种药学活性的川芎嗪是发现新的生物活性成分的良好来源,据报道，尤其是其中的柠檬苦素类化合物具有抗癌和拒食活性。为了找到更多潜在的新生物活性化合物，首先研究了nimbolinin型柠檬苦素的质谱特征。利用这些特点,总共有60种宁霉素，包括33种新化合物和至少10种生物活性化合物，通过超高效液相色谱-四极杆-飞行时间质谱(UHPLC-Q-TOF/MS)进行鉴定。此外，基于UHPLC-Q-TOF/MS和统计分析，确定了9种柠檬苦素类化合物是Toosendan和Azedarach之间的差异成分。特别是,阿好达拉果和川普果中含量较高的nimbolininA和Tosendanin（TSN）应分别是良好的标记。最后,开发了一种UHPLC-三重四极杆质谱（UHPLC-QQQ/MS）定量方法，用于nimbolininA和TSN的质量控制。本研究结果可为川芎、阿好达拉的药物开发和质量控制提供依据。
Toosendan Fructus with various pharmaceutical activities is a good source for the finding of new bioactive components, especially limonoids inside have been reported to have anticancer and antifeedant activities. To find more potential new bioactive compounds, the mass spectrometric characteristics of nimbolinin-type limonoids were first investigated. Utilizing these characteristics, totally 60 nimbolinins, including 33 new ones and at least 10 bioactive compounds, were identified by ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS). Furthermore, based on UHPLC-Q-TOF/MS and statistical analysis, 9 limonoids were identified to be the differential components between Toosendan Fructus and Azedarach Fructus. Particularly, nimbolinin A and toosendanin (TSN) with higher content in Azedarach Fructus and Toosendan Fructus respectively should be good markers. Finally, an UHPLC-triple quadrupole mass spectrometry (UHPLC-QQQ/MS) quantification approach for nimbolinin A and TSN was developed for their quality control. These results provided the basis for drug development and quality control of Toosendan Fructus and Azedarach Fructus.
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