抹茶显示出糖尿病的希望,肥胖,和肠道微生物群失调。研究表明,肠道微生物群之间存在重要联系,代谢物,和肥胖。因此,抹茶可能通过调节肠道微生物群和代谢产物对肥胖产生积极影响。这项研究使用16SrDNA测序和非靶向代谢组学来检查小鼠的盲肠内容物。通过相关性分析,我们探讨了抹茶对肥胖产生积极影响的潜在机制。结果表明,抹茶对高脂饮食(HFD)对小鼠多种生理指标的不利影响具有缓解作用,包括体重,脂肪组织重量,血清总胆固醇(TC),低密度脂蛋白(LDL)水平,以及葡萄糖耐量。此外,观察到抹茶对肠道微生物群和肠道代谢产物的结构组成有影响。具体来说,抹茶能够逆转某些改善肥胖的细菌数量的变化,比如Alloprevotella,肠杆菌,还有Rikenella,以及丰富的肥胖促进细菌Romboutsia,由HFD诱导。此外,抹茶可以影响代谢物的水平,包括formononetin,谷氨酸,焦谷氨酸,和牛磺鹅脱氧胆酸盐,在胃肠道内。此外,抹茶增强咖啡因代谢和KEGG途径中的HIF-1信号通路。相关性分析结果表明,海蒙素,可可碱,1,3,7-三甲基尿酸,维生素C与肥胖表型和已知会加剧肥胖的微生物群呈负相关,同时证明与缓解肥胖的微生物区系呈正相关。然而,谷氨酸,焦谷氨酸,牛磺鹅去氧胆酸盐具有相反的效果。总之,抹茶对肠道代谢物的影响可能归因于其对Alloprevotella丰度的调节,肠杆菌,Rikenella,和胃肠道内的Romboutsia,从而可能有助于改善肥胖。
Matcha shows promise for diabetes, obesity, and gut microbiota disorders. Studies suggest a significant link between gut microbiota, metabolites, and obesity. Thus, matcha may have a positive impact on obesity by modulating gut microbiota and metabolites. This study used 16S rDNA sequencing and untargeted metabolomics to examine the cecal contents in mice. By correlation analysis, we explored the potential mechanisms responsible for the positive effects of matcha on obesity. The results indicated that matcha had a mitigating effect on the detrimental impacts of a high-fat diet (HFD) on multiple physiological indicators in mice, including body weight, adipose tissue weight, serum total cholesterol (TC), and low-density lipoprotein (LDL) levels, as well as glucose tolerance. Moreover, it was observed that matcha had an impact on the structural composition of gut microbiota and gut metabolites. Specifically, matcha was able to reverse the alterations in the abundance of certain obesity-improving bacteria, such as Alloprevotella, Ileibacterium, and Rikenella, as well as the abundance of obesity-promoting bacteria Romboutsia, induced by a HFD. Furthermore, matcha can influence the levels of metabolites, including formononetin, glutamic acid, pyroglutamic acid, and taurochenodeoxycholate, within the gastrointestinal tract. Additionally, matcha enhances caffeine metabolism and the HIF-1 signaling pathway in the KEGG pathway. The results of the correlation analysis suggest that formononetin, theobromine, 1,3,7-trimethyluric acid, and Vitamin C displayed negative correlation with both the obesity phenotype and microbiota known to exacerbate obesity, while demonstrating positive correlations with microbiota that alleviated obesity. However, glutamic acid, pyroglutamic acid, and taurochenodeoxycholate had the opposite effect. In conclusion, the impact of matcha on gut metabolites may be attributed to its modulation of the abundance of Alloprevotella, Ileibacterium, Rikenella, and Romboutsia within the gastrointestinal tract, thereby potentially contributing to the amelioration of obesity.