■探讨microRNA-342-3p/Mg2+Mn2+依赖性蛋白磷酸酶1E(miR-342-3p/PPM1E)对细胞增殖的影响,迁移,和肾透明细胞癌(ccRCC)细胞的侵袭。
■搜索了基因芯片GSE12105、GSE23085、GSE66271和GSE66270,和miR-342-3p之间的关系,PPM1E,分析ccRCC的临床恶性表型。用miR-342-3p抑制剂转染ACHN和769-P细胞。miR-342-3p对细胞增殖的影响,迁移,并对入侵进行了检查。构建miR-342-3p稳定高表达的ACHN细胞系,并观察该细胞系在BALB/c裸鼠中的致瘤性。miR-342-3p与PPM1E的靶向关系通过双荧光素酶报告基因检测得到验证。用miR-342-3p模拟物和pcDNA-PPM1E质粒转染细胞,观察PPM1E是否可以逆转miR-342-3p过表达对细胞增殖的影响。迁移,和细胞的入侵。
■miR-342-3p在ccRCC中表达上调,不同T分期和G分期的肿瘤患者和不同预后的肿瘤患者之间存在显着差异(P<0.05)。miR-342-3p高表达组的总生存期明显短于低表达组(P<0.05)。与miR-NC组相比,miR-342-3p水平在抑制剂组中显著下调,细胞增殖能力和迁移侵入细胞数也显著降低(P<0.05)。与miR-NC组相比,miR-342-3p组肿瘤组织体积、质量和miR-342-3p水平显著增加,但显著降低PPM1EmRNA水平(P<0.05)。PPM1E在ccRCC中表达下调,不同M分期的肿瘤患者之间存在显著差异,N级,和G阶段,不同复发状态(P<0.05)。miR-342-3p可以靶向抑制PPM1E的表达。与miR-NC组相比,miR-342-3p组细胞增殖能力显著增强,迁移和侵袭细胞数量显著增加(P<0.05)。然而,PPM1E可逆转miR-342-3p模拟物对ccRCC细胞的促进作用(P<0.05)。
■miR-342-3p可以靶向抑制PPM1E的表达,从而促进了扩散,迁移,和ccRCC细胞的侵袭。
UNASSIGNED: To explore the effects of microRNA-342-3p/Mg2+Mn2+-dependent protein phosphatase 1E (miR-342-3p/PPM1E) on the proliferation, migration, and invasion of clear cell renal cell carcinoma (ccRCC) cells.
UNASSIGNED: The gene chips GSE12105, GSE23085, GSE66271, and GSE66270 were searched, and the relationship between miR-342-3p, PPM1E, and the clinical malignant phenotypes of ccRCC was analyzed. ACHN and 769-P cells were transfected with miR-342-3p inhibitor. The effects of miR-342-3p on cell proliferation, migration, and invasion were examined. ACHN cell line with stable and high expression of miR-342-3p was constructed, and the tumorigenicity of the cell line in BALB/c nude mice was observed. The targeted relationship between miR-342-3p and PPM1E was verified by dual-luciferase reporter gene assay. The cells were transfected with miR-342-3p mimic and pcDNA-PPM1E plasmids to observe whether PPM1E could reverse the effects of miR-342-3p overexpression on the proliferation, migration, and invasion of the cells.
UNASSIGNED: The expression of miR-342-3p was upregulated in ccRCC, and there were significant differences among patients with tumors of different T stages and G stages and those with different prognoses (P<0.05). The overall survival in the miR-342-3p high-expression group was significantly shorter than that in the low-expression group (P<0.05). Compared with those in the miR-NC group, the miR-342-3p level was significantly downregulated in the inhibitor group, and the cell proliferation ability and the numbers of migrating and invading cells were also significantly decreased (P<0.05). Compared with the miR-NC group, miR-342-3p group had significantly increased volume and mass of tumor tissues and miR-342-3p level, but significantly decreased level of PPM1E mRNA (P<0.05). The expression of PPM1E was downregulated in ccRCC, and there were significant differences among patients with tumors of different M stages, N stages, and G stages, and different recurrence statuses (P<0.05). The miR-342-3p could inhibit the expression of PPM1E in a targeted way. Compared with the miR-NC group, the miR-342-3p group had significantly increased cell proliferation ability and increased numbers of migrating and invading cells (P<0.05). However, PPM1E could reverse the promotion effect of miR-342-3p mimic on ccRCC cells (P<0.05).
UNASSIGNED: The miR-342-3p can inhibit PPM1E expression in a targeted way, and thus promotes the proliferation, migration, and invasion of ccRCC cells.