目的：评估在校准前后的循环应力下，植入物和基台之间的微空间和微渗漏。
方法：使用BioHPP聚醚醚酮（PEEK）基台的十二个螺钉固定的植入物假体（Noris牙科植入物系统有限公司，内舍,以色列）（I组）和12个带有计算机辅助设计/计算机辅助制造（CAD/CAM）铣削氧化锆基台（DentGallop，休斯顿,TX,美国）（第二组）连接到各自的植入物，并且将假体螺钉扭转至30N/cm(Noris)。使用扫描电子显微镜(SEM；TeScan，布尔诺,捷克共和国)。然后将24个样品诱导到循环应力(LokeshIndustries，浦那,印度)模拟180天的口腔应激持续时间。测量微空间(IA组和IIB组)的循环后应力。I组和II组再次改名为Ia组和IIb组用于微生物研究。将两个植入物组件浸入新鲜大豆酪蛋白消化肉汤（SCDB）（Himedia，孟买,印度），随后在开口端接种1.0µL大肠杆菌悬浮液（Himedia），并在37ºC下孵育7天。潜伏期过后,细胞活性通过铺板法测定，计算总菌落形成单位(CFU)。使用独立的T和Mann-Whitney检验评估结果。
结果：右前I组样品的种植体-基台连接处的平均和微空间为12.98µm，中心13.76µm,左前13.22µm,在第二组样本中，右前方是18.52µm,中心17.84µm，左前18.58µm.承受循环载荷后，IA组样品的垂直微间隙的平均水平为：在右前区域10.37µm，在中心9.34µm，在左前部为10.51µm，在IIB组样品中，右前部为14.59µm，中心13.39µm,左前13.8µm.独立t检验显示两组之间无显著性差异。循环加载后Ia组样品微生物渗漏的中值为30×103CFU/ml,和IIb组样品为42×103CFU/ml并且是显著的。
结论：BioHPPPEEK基台和CAD/CAM铣削氧化锆基台之间的平均微空间变化最小，在循环应力之前和之后都是微不足道的。在循环应力作用下，BioHPPPEEK基台-钛植入物界面的微生物渗漏比CAD/CAM铣削的氧化锆基台-钛植入物界面显着减少。
OBJECTIVE: To evaluate microspace and microleakage between implant and abutments subjected to pre- and post-calibrated cyclic stress.
METHODS: Twelve screw-retained implant prostheses with BioHPP polyetheretherketone (PEEK) abutment (Noris Dental Implant System Ltd., Nesher, Israel) (Group I) and 12 screw-retained implant prostheses with computer-assisted design/computer-assisted manufacturing (CAD/CAM) milled zirconia abutment (DentGallop, Houston, TX, USA) (Group II) were connected to their respective implant, and the prosthetic screw was torqued to 30N/cm (Noris). The microspace was evaluated using scanning electron microscopy (SEM; TeScan, Brno, Czech Republic). Twenty-four samples were then induced to cyclic stress (Lokesh Industries, Pune, India) simulating 180 days duration of oral stress. The microspaces (Group IA and Group IIB) were measured post-cyclic stress. Group I and II were again renamed into Group Ia and Group IIb for microbial study. Both implant assemblies were immersed in fresh soybean casein digest broth (SCDB) (Himedia, Mumbai, India) and subsequently inoculated with 1.0µL E. coli suspension (Himedia) at the open end and incubated at 37ºC for seven days. After the incubation period, cellular activity was determined by the spread plate method, and total colony-forming units (CFU) were calculated. The results were evaluated using independent T and Mann-Whitney tests.
RESULTS: Average and microspace at the implant-abutment junction of Group I samples in the front right was 12.98µm, center 13.76µm, front left 13.22µm, and in Group II samples, the front right was 18.52µm, center 17.84µm, front left 18.58µm.After being subjected to cyclic loading, the mean levels of the vertical microgap for Group IA samples were: in the front right region 10.37µm, in the center 9.34µm, in the front left 10.51µm and in Group IIB samples front right was 14.59µm, center 13.39µm, front left 13.8µm. Independent t-tests showed insignificant differences between the two groups. The median value of microbial leakage of Group Ia samples after cyclic loading was 30 x 103 CFU/ml, and Group IIb samples were 42 x 103 CFU/ml and were significant.
CONCLUSIONS: There was minimal variation in the mean microspace between the BioHPP PEEK abutment and CAD/CAM milled zirconia abutment, and it was insignificant before and after cyclic stress. BioHPP PEEK abutment-titanium implant interfaces showed significantly decreased microbial leakage than CAD/CAM milled zirconia abutment-titanium implant interfaces after cyclic stress.
PDF(Pubmed)