大多数生命形式,包括古细菌,细菌,真核生物合成多胺腐胺。虽然腐胺分布广泛,几种革兰氏阳性细菌,包括金黄色葡萄球菌(S.金黄色葡萄球菌),似乎是例外。我们在这里报道金黄色葡萄球菌菌株可以产生多胺腐胺,以及衍生物N-乙酰基-腐胺。来自美国典型培养物保藏中心(ATCC)的三株金黄色葡萄球菌,国家生物技术信息中心(NCBI)数据库中列出的一种菌株,其基因组序列是明确的,以及来自多个地理位置的单个患者的金黄色葡萄球菌诱导的脑脓肿的8株菌株进行了评估。每个菌株在严格条件下在完全化学成分确定的培养基(CDM)中生长,之后,通过质谱(MS)分析部分纯化的条件培养基(CM),数据报告为实验结果与对照的比率。我们通过使用13C/15N标记的精氨酸作为示踪剂证实了金黄色葡萄球菌对腐胺的合成。我们发现胍丁胺,N-乙酰腐胺,鸟氨酸,瓜氨酸,脯氨酸,和NH3都用源自13C/15N标记的精氨酸的重同位素标记。检查的菌株都没有产生精胺或亚精胺,但是来自ATCC或人脑脓肿的菌株产生腐胺和/或其衍生物N-乙酰基-腐胺。
Most forms of life, including the archaea, bacteria, and eukaryotes synthesize the polyamine putrescine. Although putrescine is widely distributed, several Gram-positive bacteria, including Staphylococcus aureus (S. aureus), appear to be the exceptions. We report here that strains of S. aureus can produce the polyamine putrescine, as well as the derivative N-acetyl-putrescine. Three strains of S. aureus from the American Type Culture Collection (ATCC), one strain listed in the National Center for Biotechnology Information (NCBI) database, whose genomic sequence is well defined, and well as eight strains from S. aureus-induced brain abscesses of individual patients from multiple geographic locations were evaluated. Each strain was grown in complete chemically defined medium (CDM) under stringent conditions, after which the partially purified conditioned medium (CM) was analyzed by mass spectroscopy (MS), and the data were reported as the ratio of experimental results to controls. We confirmed the synthesis of putrescine by S. aureus by using 13C/15N-labeled arginine as a tracer. We found that agmatine, N-acetyl-putrescine, ornithine, citrulline, proline, and NH3 were all labeled with heavy isotope derived from 13C/15N-labeled arginine. None of the strains examined produced spermine or spermidine, but strains from either ATCC or human brain abscesses produced putrescine and/or its derivative N-acetyl-putrescine.