小麦特别偏爱NO3-并且在高NH4浓度下显示毒性症状。增加硝酸盐供应可以缓解铵胁迫。然而,硝酸盐调节小麦根系生长以减轻铵毒性的潜在机制尚不清楚。在这项研究中,我们整合了生理和加权基因共表达网络分析(WGCNA),以确定小麦苗期硝酸盐减轻铵毒性的hub基因。5种NH4+/NO3-比值处理,包括100/0(Na),75/25(Nr1),50/50(Nr2),25/75(NR3),在这项研究中测试了0/100(Nn)。结果表明,单铵处理(Na)增加了侧根数量,但降低了根生物量。增加硝酸盐供应显着增加了根生物量。硝酸盐含量的增加降低了脱落酸(ABA)含量,增加了生长素(IAA)含量。此外,我们使用与根系生理生长指标显着相关的转录组数据确定了两个模块(蓝色和绿松石)。TraesCS6A02G178000和TraesCS2B02G056300在编码质体ATP/ADP转运蛋白和WRKY62转录因子的两个模块中被鉴定为hub基因,分别。此外,网络分析显示,在蓝色模块中,TraesCS6A02G178000与编码吲哚啉-2-酮单加氧酶的下调基因相互作用,SRG1,脱毒,和壁相关受体激酶。在绿松石模块中,TraesCS2B02G056300与编码ERD4,ERF109,CIGR2和WD40蛋白的基因高度相关,和转录因子,包括WRKY24,WRKY22,MYB30和JAMYB,这些都是通过增加硝酸盐供应上调的。这些研究表明,增加硝酸盐供应可以通过生理和分子调节网络改善根系生长并减轻铵的毒性。包括ROS,荷尔蒙串扰,和转录因子。
Wheat has a specific preference for NO3 - and shows toxicity symptoms under high NH4 + concentrations. Increasing the nitrate supply may alleviate ammonium stress. Nevertheless, the mechanisms underlying the nitrate regulation of wheat root growth to alleviate ammonium toxicity remain unclear. In this study, we integrated physiological and weighted gene co-expression network analysis (WGCNA) to identify the hub genes involved in nitrate alleviation of ammonium toxicity at the wheat seedling stage. Five NH4 +/NO3 - ratio treatments, including 100/0 (Na), 75/25 (Nr1), 50/50 (Nr2), 25/75 (Nr3), and 0/100 (Nn) were tested in this study. The results showed that sole ammonium treatment (Na) increased the lateral root number but reduced root biomass. Increasing the nitrate supply significantly increased the root biomass. Increasing nitrate levels decreased abscisic acid (ABA) content and increased auxin (IAA) content. Furthermore, we identified two modules (blue and turquoise) using transcriptome data that were significantly related to root physiological growth indicators. TraesCS6A02G178000 and TraesCS2B02G056300 were identified as hub genes in the two modules which coded for plastidic ATP/ADP-transporter and WRKY62 transcription factors, respectively. Additionally, network analysis showed that in the blue module, TraesCS6A02G178000 interacts with downregulated genes that coded for indolin-2-one monooxygenase, SRG1, DETOXIFICATION, and wall-associated receptor kinase. In the turquoise module, TraesCS2B02G056300 was highly related to the genes that encoded ERD4, ERF109, CIGR2, and WD40 proteins, and transcription factors including WRKY24, WRKY22, MYB30, and JAMYB, which were all upregulated by increasing nitrate supply. These studies suggest that increasing the nitrate supply could improve root growth and alleviate ammonium toxicity through physiological and molecular regulation networks, including ROS, hormonal crosstalk, and transcription factors.