杏(PrunusarmeniacaL.)是一种属玫瑰科的水果,风味独特,具有重要的经济和营养价值。水果中可溶性糖和有机酸的组成和含量是决定风味品质的关键因素。然而,杏中糖和酸积累的分子机制尚不清楚。我们测量了蔗糖,果糖,葡萄糖,山梨醇,淀粉,苹果酸,柠檬酸,可滴定酸,pH值,并调查了三个杏的转录组概况(高糖品种“舒尚干”,普通糖品种\'Sungold\',和低糖品种\'F43\')处于三个不同的发育阶段。研究结果表明,“舒尚干”积累了更多的蔗糖,葡萄糖,果糖,和山梨糖醇,更少的柠檬酸和可滴定酸,导致更好的风味;\'Sungold\'主要积累更多的蔗糖和更少的柠檬酸和淀粉的第二风味;和\'F43\'主要积累更多的可滴定酸,柠檬酸,和淀粉的甜度较低。我们调查了与淀粉和蔗糖代谢途径相关的DEGs,柠檬酸盐循环途径,糖酵解途径,和一些糖转运蛋白,它们被认为是糖和酸积累的重要调节剂。此外,对加权基因共表达网络的分析揭示了棕色模块和蔗糖之间的强大相关性,葡萄糖,和果糖,VIP被鉴定为与四种糖转运蛋白相互作用的hub基因(SLC35B3,SLC32A,SLC2A8和SLC2A13),以及糖和酸代谢的三个结构基因(MUR3、E3.2.1.67和CSLD)。此外,我们发现了一些调节这些基因的lncRNAs和miRNAs。我们的发现为糖代谢相关的功能基因提供了线索,为今后高糖杏的选育和栽培奠定基础。
The apricot (Prunus armeniaca L.) is a fruit that belongs to the Rosaceae family; it has a unique flavor and is of important economic and nutritional value. The composition and content of soluble sugars and organic acids in fruit are key factors in determining the flavor quality. However, the molecular mechanism of sugar and
acid accumulation in apricots remains unclear. We measured sucrose, fructose, glucose, sorbitol, starch, malate, citric
acid, titratable
acid, and pH, and investigated the transcriptome profiles of three apricots (the high-sugar cultivar \'Shushanggan\', common-sugar cultivar \'Sungold\', and low-sugar cultivar \'F43\') at three distinct developmental phases. The findings indicated that \'Shushanggan\' accumulates a greater amount of sucrose, glucose, fructose, and sorbitol, and less citric acid and titratable
acid, resulting in a better flavor; \'Sungold\' mainly accumulates more sucrose and less citric
acid and starch for the second flavor; and \'F43\' mainly accumulates more titratable
acid, citric
acid, and starch for a lesser degree of sweetness. We investigated the DEGs associated with the starch and sucrose metabolism pathways, citrate cycle pathway, glycolysis pathway, and a handful of sugar transporter proteins, which were considered to be important regulators of sugar and acid accumulation. Additionally, an analysis of the co-expression network of weighted genes unveiled a robust correlation between the brown module and sucrose, glucose, and fructose, with VIP being identified as a hub gene that interacted with four sugar transporter proteins (SLC35B3, SLC32A, SLC2A8, and SLC2A13), as well as three structural genes for sugar and acid metabolism (MUR3, E3.2.1.67, and CSLD). Furthermore, we found some lncRNAs and miRNAs that regulate these genes. Our findings provide clues to the functional genes related to sugar metabolism, and lay the foundation for the selection and cultivation of high-sugar apricots in the future.