背景:增强聚甲基丙烯酸甲酯(PMMA)牙科树脂的抗菌性能对于防止牙科手术后的继发感染至关重要。尽管有必要进行这种改进,在不损害其机械性能和细胞毒性的情况下增强PMMA的抗菌性能的普遍适用的方法仍然难以捉摸。因此,这项研究旨在通过开发和实施称为氧化锌/氧化石墨烯(ZnO/GO)纳米复合材料的复合材料来解决上述挑战,修改PMMA。
方法:通过一步程序成功合成了ZnO/GO纳米复合材料,并通过TEM充分表征,EDS,FTIR和XRD。然后通过吸水率和溶解度测试评价了ZnO/GO纳米复合材料改性PMMA的物理力学性能,接触角试验,三点弯曲试验,和压缩测试。此外,通过直接显微镜菌落计数法评价了改性PMMA的生物学特性,结晶紫染色和CCK-8。
结果:结果表明,成功构建了ZnO/GO纳米复合材料。当纳米复合材料在PMMA中的浓度为0.2wt。%,树脂的弯曲强度提高了23.4%,抗压强度提高了31.1%,菌落数减少60.33%。同时,发现树脂的老化并不影响其抗菌性能,CCK-8显示修饰的PMMA没有细胞毒性。
结论:ZnO/GO纳米复合材料有效地改善了PMMA的抗菌性能。此外,通过在较低的浓度范围内添加ZnO/GO纳米复合材料,树脂的力学性能得到改善。
BACKGROUND: Enhancing the antibacterial properties of polymethyl methacrylate (PMMA) dental resins is crucial in preventing secondary infections following dental procedures. Despite the necessity for such improvement, a universally applicable method for augmenting the antibacterial properties of PMMA without compromising its mechanical properties and cytotoxicity remains elusive. Consequently, this study aims to address the aforementioned challenges by developing and implementing a composite material known as zinc oxide/graphene oxide (ZnO/GO) nanocomposites, to modify the PMMA.
METHODS: ZnO/GO nanocomposites were successfully synthesized by a one-step procedure and fully characterized by TEM, EDS, FTIR and XRD. Then the physical and mechanical properties of PMMA modified by ZnO/GO nanocomposites were evaluated through water absorption and solubility test, contact angle test, three-point bending tests, and compression test. Furthermore, the biological properties of the modified PMMA were evaluated by direct microscopic colony count method, crystal violet staining and CCK-8.
RESULTS: The results revealed that ZnO/GO nanocomposites were successfully constructed. When the concentration of nanocomposites in PMMA was 0.2 wt. %, the flexural strength of the resin was increased by 23.4%, the compressive strength was increased by 31.1%, and the number of bacterial colonies was reduced by 60.33%. Meanwhile, It was found that the aging of the resin did not affect its antibacterial properties, and CCK-8 revealed that the modified PMMA had no cytotoxicity.
CONCLUSIONS: ZnO/GO nanocomposites effectively improved the antibacterial properties of PMMA. Moreover, the mechanical properties of the resin were improved by adding ZnO/GO nanocomposites at a lower range of concentrations.