背景:虽然最近在健康的雄性和雌性犬中发现了泌尿生殖系统微生物群,性激素对母犬泌尿生殖道微生物组的影响尚不清楚。更深入地了解泌尿和阴道微生物群的周期性变化将使我们能够比较健康狗的细菌种群,并评估微生物群对各种泌尿生殖道疾病的影响。因此,这项研究的目的是表征和比较健康雌性狗在发情周期不同阶段的泌尿生殖微生物群。DNA提取,16SrDNA文库的制备,进行测序和信息学分析,以确定10只健康比格犬在发情周期的每个阶段的阴道和尿液微生物群。
结果:在不同周期阶段,尿液微生物群的α和β多样性没有显着差异。同样,阿尔法多样性,在整个周期中,阴道细菌种群的丰富度和均匀度没有显着差异。然而,不同周期之间的阴道β多样性存在显着差异,除了在发情期和动情期之间.
结论:这项研究强烈表明,雌激素会影响健康雌性犬的阴道微生物群的丰度,但似乎不会影响尿液微生物组。此外,我们的数据有助于更深入地了解健康母犬的天然泌尿和阴道微生物群.
BACKGROUND: While the urogenital microbiota has recently been characterized in healthy male and female dogs, the influence of sex hormones on the urogenital microbiome of bitches is still unknown. A deeper understanding of the cyclic changes in urinary and vaginal microbiota would allow us to compare the bacterial populations in healthy dogs and assess the impact of the microbiome on various urogenital diseases. Therefore, the aim of this study was to characterize and compare the urogenital microbiota during different phases of the estrous cycle in healthy female dogs. DNA extraction, 16 S rDNA library preparation, sequencing and informatic analysis were performed to determine the vaginal and urinary microbiota in 10 healthy beagle dogs at each phase of the estrous cycle.
RESULTS: There were no significant differences in alpha and beta diversity of the urinary microbiota across the different cycle phases. Similarly, alpha diversity, richness and evenness of vaginal bacterial populations were not significantly different across the cycle phases. However, there were significant differences in vaginal beta diversity between the different cycle phases, except for between anestrus and diestrus.
CONCLUSIONS: This study strongly suggests that estrogen influences the abundance of the vaginal microbiota in healthy female dogs, but does not appear to affect the urinary microbiome. Furthermore, our data facilitate a deeper understanding of the native urinary and vaginal microbiota in healthy female dogs.