钩端螺旋体病是由钩端螺旋体属细菌引起的一种新兴的人畜共患疾病。然而,致病性和非致病性钩端螺旋体适应的调节机制和途径。在不同的环境条件下仍然难以捉摸。钩端螺旋体是钩端螺旋体的非致病性物种,仅生活在自然环境中。它不仅是探索钩端螺旋体物种环境生存的分子机制,而且是鉴定钩端螺旋体致病物种特有的毒力因子的理想模型。在这项研究中,我们的目标是通过差异RNA-seq(dRNA-seq)和小RNA-seq(sRNA-seq)分析,建立生长到指数期和固定期的L.biflexaserovarPatoc的转录起始位点(TSS)景观和小RNA(sRNA)谱。分别。我们的dRNA-seq分析发现了总共2726个TSS,也用于识别其他元素,例如,启动子和非翻译区(UTR)。此外,我们的sRNA-seq分析显示总共603个sRNA候选物,包含16个启动子相关的sRNAs,1845个UTR衍生的sRNAs,230个真正的基因间sRNAs,1365个UTR-反义sRNAs,和130个开放阅读框(ORF)-反义sRNAs。总之,这些发现反映了在不同生长条件下毕福来乳杆菌的转录复杂性,有助于我们理解毕福来乳杆菌的调控网络。据我们所知,这是第一项报告比福拉的TSS景观的研究。Biflexa的TSS和sRNA景观也可以与其致病性对应物进行比较,例如,L.borgpetersenii和L.interrogans,识别有助于其环境生存和毒力的特征。
Leptospirosis is an emerging zoonotic disease caused by bacterial species of the genus Leptospira. However, the regulatory mechanisms and pathways underlying the adaptation of pathogenic and non-pathogenic Leptospira spp. in different environmental conditions remain elusive. Leptospira biflexa is a non-pathogenic species of Leptospira that lives exclusively in a natural environment. It is an ideal model not only for exploring molecular mechanisms underlying the environmental survival of Leptospira species but also for identifying virulence factors unique to Leptospira\'s pathogenic species. In this study, we aim to establish the transcription start site (TSS) landscape and the small RNA (sRNA) profile of L. biflexa serovar Patoc grown to exponential and stationary phases via differential RNA-seq (dRNA-seq) and small RNA-seq (sRNA-seq) analyses, respectively. Our dRNA-seq analysis uncovered a total of 2726 TSSs, which are also used to identify other elements, e.g., promoter and untranslated regions (UTRs). Besides, our sRNA-seq analysis revealed a total of 603 sRNA candidates, comprising 16 promoter-associated sRNAs, 184 5\'UTR-derived sRNAs, 230 true intergenic sRNAs, 136 5\'UTR-antisense sRNAs, and 130 open reading frame (ORF)-antisense sRNAs. In summary, these findings reflect the transcriptional complexity of L. biflexa serovar Patoc under different growth conditions and help to facilitate our understanding of regulatory networks in L. biflexa. To the best of our knowledge, this is the first study reporting the TSS landscape of L. biflexa. The TSS and sRNA landscapes of L. biflexa can also be compared with its pathogenic counterparts, e.g., L. borgpetersenii and L. interrogans, to identify features contributing to their environmental survival and virulence.