背景:磷脂酰乙醇16:0/18:1(PEth),在全血中发现,是高度敏感的酒精消费生物标志物,特异性,和一个长的检测窗口。TASSO-M20装置用于从上臂自我收集毛细血管血液,与手指针刺方法相比具有优势。本研究的目的是(1)使用TASSO-M20装置验证PEth测量,(2)描述TASSO-M20在虚拟干预期间自我采血,和(3)表征PEth,随着时间的推移,单个参与者的尿乙基葡糖苷酸(uEtG)和自我报告的酒精。
方法:将在TASSO-M20塞子上干燥的血液样品中的PEth水平与(1)液体全血(N=14)和(2)干燥的血液斑点卡(DBS;N=23)中的PEth水平进行了比较。此外,自我报告的饮酒,阳性或阴性的uEtG结果(浸渍卡截止值≥300ng/mL),在对单个应急管理参与者进行虚拟访谈期间,随着时间的推移,观察到使用TASSO-M20设备对Peth水平的自我采血。使用具有串联质谱检测的高效液相色谱法来测量两种制剂的PEth水平。
结果:TASSO-M20塞子上的干血和液体全血的PEth浓度相关(0至1700ng/mL;N=14;r2=0.988;斜率=0.951),并且在浓度较低的样品亚组中(N=7;0至200ng/mL;r2=0.944,斜率=0.816)。TASSO-M20塞子和DBS上干燥血液的PEth浓度相关(0至2200ng/mL;N=23;r2=0.927;斜率=0.667),并且在浓度较低的样品亚组中(N=16;0至180ng/mL;r2=0.978,斜率=0.749)。应急管理参与者的结果表明,PEth水平(TASSO-M20)和uEtG浓度的变化彼此一致,并且与自我报告的酒精使用变化一致。
结论:我们的数据支持该实用程序,准确度,以及在虚拟研究中使用TASSO-M20设备进行血液自采集的可行性。TASSO-M20设备与典型的手指针刺方法相比具有多种优势,包括持续的采血,参与者的可接受性,可接受性访谈表明的不适感较少。
Phosphatidylethanol 16:0/18:1 (PEth), found in whole blood, is a biomarker for alcohol consumption with high sensitivity, specificity, and a long detection window. The TASSO-M20 device is used to self-collect capillary blood from the upper arm and has advantages over finger stick methods. The purpose of this study was to (1) validate PEth measurement using the TASSO-M20 device, (2) describe the TASSO-M20 for blood self-collection during a virtual intervention, and (3) characterize PEth, urinary ethyl glucuronide (uEtG) and self-reported alcohol in a single participant over time.
PEth levels in blood samples dried on TASSO-M20 plugs were compared to those in (1) liquid whole blood (N = 14) and (2) dried blood spot cards (DBS; N = 23). Additionally, the self-reported drinking, positive or negative uEtG results (dip card cutoff ≥300 ng/mL), and observed self-collection of blood with TASSO-M20 devices for PEth levels were obtained over time during virtual interviews of a single contingency management participant. High-performance liquid chromatography with tandem mass spectrometry detection was used to measure PEth levels for both preparations.
PEth concentrations from dried blood on TASSO-M20 plugs and liquid whole blood were correlated (0 to 1700 ng/mL; N = 14; r2 = 0.988; slope = 0.951) and in a subgroup of samples with lower concentrations (N = 7; 0 to 200 ng/mL; r2 = 0.944, slope = 0.816). PEth concentrations from dried blood on TASSO-M20 plugs and DBS were correlated (0 to 2200 ng/mL; N = 23; r2 = 0.927; slope = 0.667) and in a subgroup of samples with lower concentrations (N = 16; 0 to 180 ng/mL; r2 = 0.978, slope = 0.749). Results of the contingency management participant indicate that changes in PEth levels (TASSO-M20) and uEtG concentrations were consistent with each other and with changes in self-reported alcohol use.
Our data support the utility, accuracy, and feasibility of using the TASSO-M20 device for blood self-collection during a virtual study. The TASSO-M20 device had multiple advantages over the typical finger stick method, including consistent blood collection, participant acceptability, and less discomfort as indicated by acceptability interviews.