这项研究的目的是比较在进行选择性卵巢子宫切除术(OHE)或去势的猫中使用20G针从颈静脉和使用22G针从内侧隐静脉收集的样品的粘弹性测试结果。
在一项前瞻性研究中纳入了40只明显健康的猫(20只雄性和20只雌性),这些猫表现为选择性OHE或去势,观察了从中央和外周收集的全血的粘弹性测试结果。猫在采血过程中使用包括丁丙诺啡在内的标准化方案进行麻醉,氯胺酮,右美托咪定和异氟烷。几乎同时收集来自颈静脉和隐静脉的血液样本。使用定点护理设备测量血块时间(CT)进行全血的粘弹性评估,凝块形成时间(CFT),α角(α),最大凝块形成(MCF),和振幅在10和20分钟(分别为A10和A20)。在凝块时间后继续进行粘弹性测定以确定30和45分钟时的溶解指数(分别为LI30和LI45)以评估纤维蛋白溶解。
研究猫的中位年龄为18个月(5个月至5岁),中位体重为3.6kg(2.7-5.9kg)。总共有80个样品可用于分析。虽然裂解指数没有不同,凝血的粘弹性测量值在采样部位之间有所不同(CT,P<0.005;CFT,P=0.01;α,P<0.05;MCF,P<0.0005;A10,P<0.0005;A20,P<0.0005)。
颈静脉血液样本的粘弹性结果似乎比从隐内侧静脉收集的结果更容易高凝。建议应始终使用同一站点进行串行监测或收集研究数据。
The aim of this study was to compare viscoelastic test results from samples collected from a jugular vein using a 20 G needle and a medial
saphenous vein using a 22 G needle in cats presenting for elective ovariohysterectomy (OHE) or castration.
Forty apparently healthy cats (20 males and 20 females) presenting for elective OHE or castration were included in a prospective study observing viscoelastic test results from central and peripherally collected whole blood. Cats were anesthetized during blood collection with a standardized protocol including buprenorphine, ketamine, dexmedetomidine and isoflurane. Blood samples from jugular and
saphenous veins were collected near simultaneously. Viscoelastic evaluations of whole blood were performed using a point-of-care device measuring clot time (CT), clot formation time (CFT), alpha angle (α), maximum clot formation (MCF), and amplitude at 10 and 20 mins (A10 and A20, respectively). Viscoelastometry continued post-clot time to determine a lysis index at 30 and 45 mins (LI30 and LI45, respectively) to assess fibrinolysis.
Studied cats had a median age of 18 months (range 5 months to 5 years) and a median weight of 3.6 kg (range 2.7-5.9 kg). A total of 80 samples were available for analysis. While lysis indices were not different, viscoelastic measures of coagulation differed between sampling sites (CT, P <0.005; CFT, P = 0.01; α, P <0.05; MCF, P <0.0005; A10, P <0.0005; A20, P <0.0005).
Viscoelastic results from jugular venous blood samples appear to be more hypercoagulable than those collected from the medial
saphenous vein, suggesting that the same site should be used consistently for serial monitoring or for collecting study data.