我在睡眠和昼夜节律神经生物学实验室学习,精神病学和行为科学系,斯坦福大学医学院,从2018年4月到2020年3月。在斯坦福大学,我主要研究了以下主题:(1)使用小鼠的睡眠基础研究(对小鼠施用具有睡眠或觉醒作用的化合物并检查其作用),(2)昼夜节律紊乱的研究。世界上只有少数机构能够用老鼠进行睡眠基础研究,斯坦福大学是一个让自己沉浸在研究中的好环境,因为它不仅是精神科医生的家,也是神经学家和许多基础研究人员的家。在这篇文章中,我想回顾一下我在国外学习期间进行的实验,使用小鼠来验证天然化合物对觉醒或睡眠的影响。在一项研究中,我们评估了银杏内酯的作用(A,B,和C)和白果内酯在唤醒时,运动,和核心体温。结果表明,在小鼠生理性睡眠-觉醒周期中,只有银杏内酯B剂量依赖性地增加唤醒量,减少NREM睡眠量。在另一项研究中,我们在急性失眠模型下测试了清酒酵母对小鼠的睡眠诱导作用。我们发现清酒酵母剂量依赖性地增加了REM和非REM睡眠,口服清酒酵母后6小时内唤醒减少,在新的笼子里运动和核心体温降低。
I studied at the Sleep and Circadian Neurobiology Laboratory, Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, from April 2018 to March 2020. At Stanford University, I mainly researched the following themes: (1) sleep basic research using mice (administering compounds with sleep or wakefulness effects to mice and examining their effects), and (2) research on circadian rhythm disorders. There are only a few institutions in the world that can conduct sleep basic research using mice, and Stanford University is a wonderful environment to immerse yourself in research, as it is home to not only psychiatrists but also neurologists and many basic researchers. In this article, I would like to review the experiments I conducted during my study abroad, using mice to verify the effects of natural compounds on wakefulness or sleep. In one study, we evaluated the effects of ginkgolides (A, B, and C) and bilobalide on arousal, locomotion, and core body temperature. The results showed that only ginkgolide B dose-dependently increased the amount of arousal and decreased the amount of NREM sleep in the physiological sleep-wake cycle of mice. In another study, we tested the sleep-inducing effects of sake yeast in mice under an acute insomnia model. We showed that sake yeast dose-dependently increased REM and non-REM sleep, decreased arousal within 6 hours after oral administration of sake yeast, and decreased locomotion and core body temperature in a new cage.