细菌纤维素(BC)是一种以其独特的物理化学和机械属性而闻名的天然聚合物,包括显著的持水能力,结晶度和原始的纤维网络结构。虽然BC在农业领域有着广泛的应用,工业,和医学,其工业利用受到生产成本和产量限制的阻碍。在这项研究中,根瘤菌sp.从豆根中分离,并在最佳条件下系统地评估BC合成,与Komagataeibacterhansenii产生的BC进行比较分析。研究表明,根瘤菌。当提供1.5%的葡萄糖碳源和0.15%的酵母提取物氮源时,表现出最佳的BC合成。在30°C和pH6.5的静态条件下,确定了最有利于生长和BC产生的条件(2.5g/L)。使用乳链菌肽引入修饰以增强BC特性,并通过各种技术对所得的BC-Nisin复合材料进行了全面表征,包括FE-SEM,FTIR,孔隙度,肿胀,过滤,和抗菌活性评估。结果表明,根瘤菌产生的BC。显示出与K.hansenii生产的BC相当的性能。此外,BC-乳酸链球菌素复合物对大肠杆菌和铜绿假单胞菌具有显著的抑制活性。这项研究为BC的生产提供了有价值的见解,修改,并利用根瘤菌进行表征。,突出了使其在不同应用中有效的特殊属性。
Bacterial cellulose (BC) is a natural polymer renowned for its unique physicochemical and mechanical attributes, including notable water-holding capacity, crystallinity, and a pristine fiber network structure. While BC has broad applications spanning agriculture, industry, and medicine, its industrial utilization is hindered by production costs and yield limitations. In this study,
Rhizobium sp. was isolated from bean roots and systematically assessed for BC synthesis under optimal conditions, with a comparative analysis against BC produced by Komagataeibacter hansenii. The study revealed that
Rhizobium sp. exhibited optimal BC synthesis when supplied with a 1.5% glucose carbon source and a 0.15% yeast extract nitrogen source. Under static conditions at 30 °C and pH 6.5, the most favorable conditions for growth and BC production (2.5 g/L) were identified. Modifications were introduced using nisin to enhance BC properties, and the resulting BC-nisin composites were comprehensively characterized through various techniques, including FE-SEM, FTIR, porosity, swelling, filtration, and antibacterial activity assessments. The results demonstrated that BC produced by
Rhizobium sp. displayed properties comparable to K. hansenii-produced BC. Furthermore, the BC-nisin composites exhibited remarkable inhibitory activity against Escherichia coli and Pseudomonas aeruginosa. This study contributes valuable insights into BC\'s production, modification, and characterization utilizing
Rhizobium sp., highlighting the exceptional properties that render it efficacious across diverse applications.