■为了研究干细胞因子(SCF)/c-KIT的眼内浓度分布,半乳糖凝集素-1(GAL-1),和血管内皮生长因子(VEGF)-A关于视网膜疾病和治疗反应。
■研究组包括13例年龄相关性黄斑变性(AMD)患者,196例新生血管性AMD(nAMD),21患有糖尿病性黄斑水肿(DME),10患有视网膜静脉阻塞(RVO),和34名患有白内障的正常人。SCF的房水水平,c-KIT,根据疾病组和治疗反应,通过免疫测定分析GAL-1和VEGF-A。
■SCF的水含量增加,c-KIT,在nAMD眼中观察到GAL-1(2.67±3.66,296.84±359.56和3945.61±5976.2pg/mL,分别),DME(1.64±0.89,238.80±265.54和3701.23±4340.54pg/mL,分别),和RVO(4.62±8.76,509.63±647.58和9079.60±11909.20pg/mL,分别)与对照组(1.13±0.24、60.00±0.00和613.27±1595.12pg/mL,分别)。在nAMD的眼中,三种细胞因子水平均与VEGF-A水平呈正相关。玻璃体内注射抗VEGF药物后,GAL-1和VEGF-A水平明显下降,而SCF和c-Kit无明显变化。治疗后视力改善的nAMD患者的眼c-KIT水平明显降低,基线时的GAL-1和VEGF-A。
■nAMD患者眼内细胞因子水平显著升高,DME,和RVO与对照组相比,它们对抗VEGF治疗表现出不同的反应。根据这一结果及其与血管生成的已知关联,这些细胞因子可能是未来研究的潜在治疗靶点。
UNASSIGNED: To investigate the intraocular concentration profiles of stem cell factor (SCF)/c-KIT, galectin-1 (GAL-1), and vascular endothelial growth factor (VEGF)-A with regard to retinal disease and treatment response.
UNASSIGNED: The study group included 13 patients with dry age-related macular degeneration (AMD), 196 with neovascular AMD (nAMD), 21 with diabetic macular edema (DME), 10 with retinal vein occlusion (RVO), and 34 normal subjects with cataracts. Aqueous humor levels of SCF, c-KIT, GAL-1, and VEGF-A were analyzed by immunoassay according to disease group and treatment response.
UNASSIGNED: Increased aqueous levels of SCF, c-KIT, and GAL-1 were observed in eyes with nAMD (2.67 ± 3.66, 296.84 ± 359.56, and 3945.61 ± 5976.2 pg/mL, respectively), DME (1.64 ± 0.89, 238.80 ± 265.54, and 3701.23 ± 4340.54 pg/mL, respectively), and RVO (4.62 ± 8.76, 509.63 ± 647.58, and 9079.60 ± 11909.20 pg/mL, respectively) compared with controls (1.13 ± 0.24, 60.00 ± 0.00, and 613.27 ± 1595.12 pg/mL, respectively). In the eyes of nAMD, the levels of all three cytokines correlated positively with VEGF-A levels. After intravitreal injections of anti-VEGF agents, the levels of GAL-1 and VEGF-A decreased significantly, whereas those of SCF and c-Kit showed no significant change. Eyes of nAMD patients with improved vision after treatment had significantly lower levels of c-KIT, GAL-1, and VEGF-A at baseline.
UNASSIGNED: The intraocular levels of cytokines were significantly elevated in eyes with nAMD, DME, and RVO compared to the controls and they showed different response to anti-VEGF treatment. With this result and their known association with angiogenesis, these cytokines may be potential therapeutic targets for future research.