■神经调节蛋白-1(NRG-1)似乎在几种神经精神疾病的发病机理中起作用,包括癫痫。我们进行了一项研究,以研究抗癫痫药物对首发局灶性癫痫患者NRG-1mRNA和NRG-1蛋白水平的影响。
■NRG-1mRNA亚型的水平(I型,II,III,和IV)在39名健康对照者的外周血单核细胞(PBMC)中,39例首发局灶性癫痫患者在抗惊厥药物(ASM)治疗前和ASM给药后4周进行RT-qPCR检测,用ELISA法测定各组样品血清中NRG-1蛋白的含量。此外,疗效之间的关系,NRG-1mRNA表达,分析NRG-1蛋白表达。
■在接受ASM治疗的首发局灶性癫痫患者中,NRG-1mRNA水平逐渐升高,与用药前明显不同,但仍低于健康对照组(均P<0.001)。给药前后,NRG-1蛋白水平在癫痫患者中明显高于健康对照组,随访时间延长,未发现明显变化(P<0.001)。使用ASM的癫痫患者能够控制癫痫发作,总有效率为97.4%。NRG-1mRNA水平与疗效呈负相关:随着NRG-1mRNA水平的升高,癫痫发作减少(均P<0.05)。
■我们的研究表明NRG-1可能在癫痫的病理生理学中起作用。NRG-1mRNA可能为发现新型癫痫治疗标志物和新型ASM治疗靶点提供思路。
UNASSIGNED: Neuregulin-1 (NRG-1) appears to play a role in the pathogenesis of several neuropsychiatric disorders, including epilepsy. We conducted a study to investigate the effect of anti-seizure medication on NRG-1 mRNA and NRG-1 protein levels in patients with first-episode focal epilepsy.
UNASSIGNED: The levels of NRG-1 mRNA isoforms (type I, II, III, and IV) in peripheral blood mononuclear cells (PBMCs) of 39 healthy controls, 39 first-episode focal epilepsy patients before anti-seizure medication (ASM) therapy and four weeks after administration of ASM were measured by RT-qPCR, and the levels of NRG-1 protein in the serum of samples of each group were determined using ELISA. In addition the relationship between efficacy, NRG-1 mRNA expression, and NRG-1 protein expression was analyzed.
UNASSIGNED: The levels of NRG-1 mRNA progressively increased in patients with first-episode focal epilepsy treated with ASM and were distinctly different from those before medication, but remained lower than in healthy controls (all P < 0.001). Before and after drug administration, NRG-1 protein levels were substantially higher in epileptic patients than in healthy controls, and no significant changes were detected with prolonged follow-up (P < 0.001). Patients with epilepsy who utilized ASM were able to control seizures with an overall efficacy of 97.4%. There was a negative correlation between NRG-1 mRNA levels and efficacy: as NRG-1 mRNA levels increased, seizures reduced (all P < 0.05).
UNASSIGNED: Our research indicated that NRG-1 may play a role in the pathophysiology of epilepsy. NRG-1 mRNA may provide ideas for the discovery of novel epilepsy therapeutic markers and therapeutic targets for novel ASM.