豌豆是一种有前途的作物,有潜力提供食物和饲料,但是它的基因组学还没有得到充分的探索。确定理想性状的基因,如耐旱性和抗病性,对改善工厂至关重要。草豌豆目前缺乏已知的R基因,包括核苷酸结合位点-富含亮氨酸的重复序列(NBS-LRR)基因家族,在保护植物免受生物和非生物胁迫方面起着关键作用。在我们的研究中,我们使用最近发表的草豌豆基因组和可用的转录组数据来鉴定274个NBS-LRR基因.所报道的植物上的分类基因与LsNBS之间的进化关系表明,124个基因具有TNL结构域,而150个基因有CNL结构域。所有基因都含有外显子,范围从1到7。鉴定出长度为16至30个氨基酸的十个保守基序。我们在132个LsNBS中发现了含TIR域的基因,在84个LsNBS中使用63个TIR-1和69个TIR-2以及RX-CCLike。我们还确定了几个受欢迎的图案,包括P循环,Uup,激酶-GTP酶,ABC,ChvD,CDC6,Rnase_H,Smc,CDC48和SpoVK。根据基因富集分析,鉴定的基因经历了几种生物过程,如植物防御,先天免疫,水解酶活性,和DNA结合。在上游地区,确定了103个转录因子,它们控制着影响植物水杨酸排泄的附近基因的转录,茉莉酸甲酯,乙烯,和脱落酸。根据RNA-Seq表达分析,85%的编码基因具有高表达水平。在盐胁迫条件下选择9个LsNBS基因用于qPCR。大多数基因在50和200μMNaCl下显示出上调。然而,LsNBS-D18、LsNBS-D204和LsNBS-D180与它们各自的表达水平相比显示出降低或显著的下调,为LsNBS在盐胁迫条件下的潜在功能提供了进一步的见解。它们为LsNBS在盐胁迫条件下的潜在功能提供了有价值的见解。我们的发现还揭示了豆科植物中NBS-LRR基因的进化和分类。突出草豌豆的潜力。进一步的研究可以集中在这些基因的功能分析上,以及它们在育种计划中提高盐度的潜在用途,干旱,和这种重要作物的抗病性。
Grass pea is a promising crop with the potential to provide food and fodder, but its genomics has not been adequately explored. Identifying genes for desirable traits, such as drought tolerance and disease resistance, is critical for improving the plant. Grass pea currently lacks known R-genes, including the nucleotide-binding site-leucine-rich repeat (
NBS-LRR) gene family, which plays a key role in protecting the plant from biotic and abiotic stresses. In our study, we used the recently published grass pea genome and available transcriptomic data to identify 274
NBS-LRR genes. The evolutionary relationships between the classified genes on the reported plants and LsNBS revealed that 124 genes have TNL domains, while 150 genes have CNL domains. All genes contained exons, ranging from 1 to 7. Ten conserved motifs with lengths ranging from 16 to 30 amino acids were identified. We found TIR-domain-containing genes in 132 LsNBSs, with 63 TIR-1 and 69 TIR-2, and RX-CCLike in 84 LsNBSs. We also identified several popular motifs, including P-loop, Uup, kinase-GTPase, ABC, ChvD, CDC6, Rnase_H, Smc, CDC48, and SpoVK. According to the gene enrichment analysis, the identified genes undergo several biological processes such as plant defense, innate immunity, hydrolase activity, and DNA binding. In the upstream regions, 103 transcription factors were identified that govern the transcription of nearby genes affecting the plant excretion of salicylic acid, methyl jasmonate, ethylene, and abscisic acid. According to RNA-Seq expression analysis, 85% of the encoded genes have high expression levels. Nine LsNBS genes were selected for qPCR under salt stress conditions. The majority of the genes showed upregulation at 50 and 200 μM NaCl. However, LsNBS-D18, LsNBS-D204, and LsNBS-D180 showed reduced or drastic downregulation compared to their respective expression levels, providing further insights into the potential functions of LsNBSs under salt stress conditions. They provide valuable insights into the potential functions of LsNBSs under salt stress conditions. Our findings also shed light on the evolution and classification of
NBS-LRR genes in legumes, highlighting the potential of grass pea. Further research could focus on the functional analysis of these genes, and their potential use in breeding programs to improve the salinity, drought, and disease resistance of this important crop.