未经证实:海水溺水引起的急性肺损伤(ALI)是一种严重的临床疾病,其特征是肺泡毛细血管通透性增加,过度的炎症反应,和难治性低氧血症。C-藻蓝蛋白(C-PC),一种在蓝绿藻如螺旋藻中发现的胆红素,由于其有益的药理作用,被广泛用于食品和膳食营养补充剂领域。以前的研究表明,C-PC具有抗炎作用,抗氧化剂,和抗凋亡活性。
UNASSIGNED:因此,本研究探讨了C-PC对脂多糖(LPS)和海水(SW)诱导的ALI(SW和LPS诱导的ALI)的保护作用和潜在机制。
UNASSIGNED:通过气管内施用5mg/kgLPS和25%SW来建立ALI小鼠的SW和LPS小鼠模型。通过腹膜内注射给予不同剂量的C-PC(100、200和400mg/kg)7天。此外,在用C-PC(120μg/ml)处理后用25%SW和10μg/mlLPS刺激后,测定RAW264.7和MLE-12细胞的间隙连接通讯。此外,动脉氧分压,肺湿/干重比,支气管肺泡灌洗液(BALF)中的总蛋白含量和MPO水平,并对肺组织进行组织病理学和超微结构染色。氧化应激指数,促炎介质的水平,上皮细胞活力和凋亡,研究了C-PC对NF-κB/NLRP3轴的调节作用。
未经证实:结果显示C-PC可显著减轻病理性损伤,抑制氧化应激,炎症和细胞凋亡,并增强肺组织中上皮细胞的活力。此外,显示C-PC抑制NF-κB/NLRP3途径的激活和NLRP3炎性体复合物的形成。
未经批准:总而言之,C-PC在SW和LPS诱导的ALI/ARDS中显示出有希望的治疗价值,为ALI/ARDS治疗提供新的见解。
UNASSIGNED: Seawater drowning-induced acute lung injury (ALI) is a severe clinical condition characterized by increased alveolar-capillary permeability, excessive inflammatory response, and refractory hypoxemia. C-phycocyanin (C-PC), a biliprotein found in blue-green algae such as spirulina platensis, is widely used in the food and dietary nutritional supplement fields due to its beneficial pharmacological effects. Previous studies have revealed that C-PC has anti-inflammatory, antioxidant, and anti-apoptotic activities.
UNASSIGNED: Therefore, this study investigated the protective effect and underlying mechanisms of C-PC on lipopolysaccharide (LPS) and seawater (SW) induced ALI (SW and LPS-induced ALI).
UNASSIGNED: An SW and LPS mouse model of ALI mice was established through intratracheal administration of 5mg/kg LPS and 25% SW. Different doses of C-PC (100, 200 and 400 mg/kg) were administered by intraperitoneal injection for seven days. In addition, gap junction communication in RAW264.7 and MLE-12 cells was determined following stimulation with 25% SW and 10 μg/ml LPS after treatment with C-PC (120 μg/ml). Moreover, the arterial partial pressure of oxygen, lung wet/dry weight ratios, total protein content and MPO levels in the bronchoalveolar lavage fluid (BALF), and the histopathologic and ultrastructure staining of the lung tissues were determined. The oxidative stress index, levels of the pro-inflammatory mediators, epithelial cell viability and apoptosis, and the regulatory effect of C-PC on the NF-κB/NLRP3 axis were investigated.
UNASSIGNED: The results showed that C-PC significantly alleviated pathological damages, suppressed oxidative stress, inflammation and apoptosis, and enhanced the viability of epithelial cells in the lung tissues. Furthermore, C-PC was shown to inhibit activation of the NF-κB/NLRP3 pathway and the formation of the NLRP3 inflammasome complex.
UNASSIGNED: In conclusion, C-PC shows promising therapeutic value in SW and LPS-induced ALI/ARDS, providing new insight into ALI/ARDS treatment.