膀胱癌和骨肉瘤是源自膀胱内上皮组织和骨或肌肉组织的两种类型的癌症。超声引导活检为膀胱癌和骨肉瘤的诊断和治疗提供了至关重要的支持。然而,肌球蛋白轻链激酶(MYLK)和caldesmon(CALD1)与膀胱癌和骨肉瘤的关系尚不清楚.膀胱癌数据集GSE65635和GSE100926,骨肉瘤数据集GSE39058从基因表达综合获得。筛选差异表达基因(DEGs)并进行加权基因共表达网络分析。蛋白质相互作用网络的构建与分析,功能富集分析,基因集富集分析。绘制基因表达热图并进行免疫浸润分析。进行比较毒性基因组学数据库分析以发现与核心基因最相关的疾病。进行蛋白质印迹实验。TargetScan筛选了调节中央DEG的miRNA。我们获得了54度。功能富集分析显示细胞分化方面的显著富集,软骨发育,骨骼发育,肌肉肌动蛋白细胞骨架,肌动蛋白丝,RhoGTPase结合,DNA结合,成纤维细胞结合,MAPK信号通路,凋亡,和癌症途径。基因集富集分析表明,DEGs主要富集在骨骼发育方面,软骨发育,肌肉肌动蛋白细胞骨架,MAPK信号通路,和凋亡。免疫浸润分析显示,当调节性T细胞高表达时,嗜酸性粒细胞表现出相似的高表达,表明T细胞调节和嗜酸性粒细胞之间存在强正相关,这可能会影响骨肉瘤的疾病进展。我们确定了6个核心基因(SRF,CTSK,MYLK,VCAN,MEF2C,CALD1)。与正常样本相比,MYLK和CALD1与生存率显着相关,并且在膀胱癌和骨肉瘤样本中的表达较低。比较毒物基因组学数据库分析结果表明核心基因与骨肉瘤相关,膀胱肿瘤,膀胱疾病,肿瘤,炎症,和坏死。Westernblotting结果显示MYLK和CALD1在膀胱癌和骨肉瘤中的表达水平低于正常组织。MYLK和CALD1可能在调节膀胱癌和骨肉瘤的肌肉收缩和平滑肌功能中起作用。MYLK和CALD1的低表达与预后较差有关。
Bladder cancer and osteosarcoma are 2 types of cancers that originate from epithelial tissues inside the bladder and bone or muscle tissues. Ultrasound-guided biopsies provide crucial support for the diagnosis and treatment of bladder cancer and osteosarcoma. However, the relationship between myosin light chain kinase (MYLK) and caldesmon (CALD1) and bladder cancer and osteosarcoma remains unclear. The bladder cancer datasets GSE65635 and GSE100926, the osteosarcoma dataset GSE39058, were obtained from gene expression omnibus. Differentially expressed genes (DEGs) were screened and weighted gene co-expression network analysis was performed. The construction and analysis of protein-protein interaction network, functional enrichment analysis, gene set enrichment analysis. Gene expression heat map was drawn and immune infiltration analysis was performed. The comparative toxicogenomics database analysis were performed to find disease most related to core gene. Western blotting experiments were performed. TargetScan screened miRNAs that regulated central DEGs. We obtained 54 DEGs. Functional enrichment analysis revealed significant enrichment in terms of cellular differentiation, cartilage development, skeletal development, muscle actin cytoskeleton, actin filament, Rho GTPase binding, DNA binding, fibroblast binding, MAPK signaling pathway, apoptosis, and cancer pathways. Gene set enrichment analysis indicated that DEGs were primarily enriched in terms of skeletal development, cartilage development, muscle actin cytoskeleton, MAPK signaling pathway, and apoptosis. The immune infiltration analysis showed that when T cells regulatory were highly expressed, Eosinophils exhibited a similar high expression, suggesting a strong positive correlation between T cells regulatory and Eosinophils, which might influence the disease progression in osteosarcoma. We identified 6 core genes (SRF, CTSK, MYLK, VCAN, MEF2C, CALD1). MYLK and CALD1 were significantly correlated with survival rate and exhibited lower expression in bladder cancer and osteosarcoma samples compared to normal samples. Comparative toxicogenomics database analysis results indicated associations of core genes with osteosarcoma, bladder tumors, bladder diseases, tumors, inflammation, and necrosis. The results of Western blotting showed that the expression levels of MYLK and CALD1 in bladder cancer and osteosarcoma were lower than those in normal tissues. MYLK and CALD1 likely play a role in regulating muscle contraction and smooth muscle function in bladder cancer and osteosarcoma. The lower expression of MYLK and CALD1 is associated with poorer prognosis.