目的:本研究检测了两种环烯醚萜苷莫诺苷和马钱素在大鼠血浆中口服粗品和加工后的药代动力学特征。
方法:快速,建立了具有多反应监测模式的选择性和特异性的高效液相色谱/电喷雾串联质谱,以同时研究口服粗品C.officinals及其九智平后大鼠血浆中莫诺苷和马钱素的药代动力学特征。
结果:可以在7.4min内同时测定粗和加工C.officinals中的莫诺苷和马钱素。对于所有分析物在45.45-4800ng/mL的浓度范围内获得线性校准曲线。日内和日间精度相对标准偏差小于2.84%和4.12%,分别。
结论:还系统地比较了两种环烯醚萜苷的药代动力学参数。本文为进一步解释中药炮制机理提供了理论依据。
OBJECTIVE: The present study examined the pharmacokinetic profiles of two iridoid glycosides named morroniside and loganin in rat plasma after oral administration of crude and processed Cornus officinals.
METHODS: A rapid, selective and specific high-performance liquid chromatography/electrospray ionization tandem mass spectrometry with multiple reactions monitoring mode was developed to simultaneously investigate the pharmacokinetic profiles of morroniside and loganin in rat plasma after oral administration of crude C. officinals and its jiuzhipin.
RESULTS: The morroniside and loganin in crude and processed C. officinals could be simultaneously determined within 7.4 min. Linear calibration curves were obtained over the concentration ranges of 45.45-4800 ng/mL for all the analytes. The intra-and inter-day precisions relative standard deviation was lesser than 2.84% and 4.12%, respectively.
CONCLUSIONS: The pharmacokinetic parameters of two iridoid glucosides were also compared systematically between crude and processed C. officinals. This paper provides the theoretical proofs for further explaining the processing mechanism of Traditional Chinese Medicines.