背景:用于分析Western印迹图像的当前解决方案缺乏透明性和再现性,或者如果必须确保分析的再现性,则使用起来可能是乏味的。
结果:这里,我们提出了一个开源的凝胶图像分析程序,IOCBIO凝胶.它旨在简化图像分析,并将分析结果与描述测量的元数据链接起来。该软件在所有主要的桌面操作系统上运行。它允许人们在具有数据本地存储的单个研究人员环境中使用它,或者在使用中央数据库的多个研究人员环境中使用它,以促进研究团队内外的数据共享。通过记录原始图像和对其执行的所有操作,例如图像裁剪,背景减法,样品泳道选择,和整合边界,该软件确保了分析的可重复性,并简化了在研究的任何阶段进行校正。可以通过直接访问用于存储分析结果的数据库或通过导出相关数据来获得分析结果。
结论:该软件不仅限于WesternBlot图像分析,还可用于分析作为许多其他广泛使用的生化技术(例如等电聚焦)的一部分获得的图像。通过记录原始数据和所有分析步骤,该程序提高了分析的可重复性,并有助于在相关领域实施FAIR原则。
Current solutions for the analysis of Western Blot images lack either transparency and reproducibility or can be tedious to use if one has to ensure the reproducibility of the analysis.
Here, we present an open-source gel image analysis program, IOCBIO Gel. It is designed to simplify image analysis and link the analysis results with the metadata describing the measurements. The software runs on all major desktop operating systems. It allows one to use it in either a single-researcher environment with local storage of the data or in a multiple-researcher environment using a central database to facilitate data sharing within the research team and beyond. By recording the original image and all operations performed on it, such as image cropping, subtraction of background, sample lane selection, and integration boundaries, the software ensures the reproducibility of the analysis and simplifies making corrections at any stage of the research. The analysis results are available either through direct access to the database used to store it or through the export of the relevant data.
The software is not only limited to Western Blot image analysis and can be used to analyze images obtained as a part of many other widely used biochemical techniques such as isoelectric focusing. By recording the original data and all the analysis steps, the program improves reproducibility in the analysis and contributes to the implementation of FAIR principles in the related fields.