传染性造血系统坏死病毒(IHNV)是传染性造血系统坏死的病原体,其爆发是导致虹鳟鱼水产养殖重大损失的原因。世界范围内分离的IHNV菌株已分为五个主要基因组,J,E,L,M,和U.到目前为止,仅对J和M基因组进行了比较转录组分析。在这项研究中,我们比较了U基因组和J基因组IHNV感染的RTG-2细胞与模拟感染的RTG-2细胞的转录组概况。RNA-seq结果揭示了17064个新基因,其中7390个基因进行了功能注释。U和JIHNV感染细胞之间的差异表达基因(DEG)分析显示2,238DEGs,包括1,011个下调基因和1,227个上调基因。在2,238个DEG中,发现了345个新基因。DEG与免疫反应有关,细胞信号转导,并对病毒性疾病进行了进一步分析。RT-qPCR验证证实了免疫应答相关基因trpm2、sting、itgb7,ripk2和irf1,细胞信号转导相关基因irl,cacnb2,bmp2l,gadd45α,和plk2,以及病毒疾病相关基因mlf1,mtor,armc5、pik3r1和c-myc与转录组分析结果一致。一起来看,我们的发现为IHNV的U和J基因组的差异毒力提供了全面的转录分析,并为IHNV菌株的致病机制提供了新的思路。
Infectious hematopoietic necrosis virus (IHNV) is the causative pathogen of infectious hematopoietic necrosis, outbreaks of which are responsible for significant losses in rainbow trout aquaculture. Strains of IHNV isolated worldwide have been classified into five major genogroups, J, E, L, M, and U. To date, comparative transcriptomic analysis has only been conducted individually for the J and M genogroups. In this study, we compared the transcriptome profiles in U genogroup and J genogroup IHNV-infected RTG-2 cells with mock-infected RTG-2 cells. The RNA-seq results revealed 17,064 new genes, of which 7,390 genes were functionally annotated. Differentially expressed gene (DEG) analysis between U and J IHNV-infected cells revealed 2,238 DEGs, including 1,011 downregulated genes and 1,227 upregulated genes. Among the 2,238 DEGs, 345 new genes were discovered. The DEGs related to immune responses, cellular signal transduction, and viral diseases were further analyzed. RT-qPCR validation confirmed that the changes in expression of the immune response-related genes trpm2, sting, itgb7, ripk2, and irf1, cellular signal transduction-related genes irl, cacnb2, bmp2l, gadd45α, and plk2, and viral disease-related genes mlf1, mtor, armc5, pik3r1, and c-myc were consistent with the results of transcriptome analysis. Taken together, our findings provide a comprehensive transcriptional analysis of the differential virulence of the U and J genogroups of IHNV, and shed new light on the pathogenic mechanisms of IHNV strains.